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Preliminary results of screening IITA improved germplasm for resistance to Cassava Brown Streak Disease (CBSD) in Uganda, Ntawuruhunga, P., Kiryowa, M., Okechukwu, R., Otim Okello, F. and Kanju, E., in: Tropical Roots and Tuber Crops and the Challenges of Globalization and Climate Changes. Proceedings of the 11th triennial Symposium of the ISTRC-AB, 4 to 8 October 2010, pages 398-405, ISTRC-AB, Ibadan, Nigeria, 2012. |
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Preliminary results of screening IITA's improved germplasm for resistance to cassava brown streak disease (CBSD) in Uganda, Ntawuruhunga, P., Kiryowa, M., Otim Okello, F., Okechukwu, R. and Kanju, E., in: Root and tuber crops for poverty alleviation through science and technology for sustainable development: proceedings of the 10th symposium of ISTRC-AB held from 8-12 October, 2007 in Maputo, Mozambique, pages 361-362, ISTRC-AB, 2010. |
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Protection of tropical food crops by means of host plant resistance with particular emphasis on virus diseases, Rossel, H. W., in: Paper presented at International Conference on Tropical Crop Protection of the Lyon and South-East Scientific Foundation: Lyon, 8-10 July, 1981, 1981. |
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QTL associated with resistance to cassava brown streak and cassava mosaic diseases in a bi-parental cross of two Tanzanian farmer varieties, Namikonga and Albert, Masumba, E.*, Kapinga, F.*, Mkamilo, G.*, Salum, K.*, Kulembeka, H.*, Rounsley, S., Bredeson, J. V., Lyons, J. B., Rokhsar, D. S., Kanju, E., Katari, M. S., Myburg, A. A.*, van der Merwe, A.* and Ferguson, M., in: Theoretical and Applied Genetics, pages 1-22, ISSN 0040-5752, 2017. [DOI] |
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QTL mapping for pest and disease resistance in cassava and coincidence of some QTL with introgression regions derived from Manihot glaziovii, Nzuki, I., Katari, M. S., Bredeson, J. V., Masumba, E.*, Kapinga, F.*, Salum, K.*, Mkamilo, G.*, Shah, T., Lyons, J. B., Rokhsar, D. S., Rounsley, S., Myburg, A. A.* and Ferguson, M., in: Frontiers in Plant Science, volume 8, number 1168, pages 1-15, ISSN 1664-462X, 2017. [DOI] |
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QTLs associated with resistance to the cassava mosaic disease: new directions for a diverse planet, Lokko, Y., Gedil, M. and Dixon, A., in: Proceedings of the 4th International Crop Science Congress, 26 September-1 October 2004, Brisbane, Australia, 2004. |
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QTLs for resistance to the cassava mosaic disease in African cassava accessions, Lokko, Y., Gedil, M. and Dixon, A., in: Book of Abstracts of the 11th International Plant and Animal Genome Conference (PAG XII), 10-14 January 2004, San Diego CA, USA, 2004. |
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Rapid and mass screening of banana and plantain for resistance to Black sigatoka disease using detached leaf and in vitro plantlets, Ojiambo, P., Twizeyimana, M., Tenkouano, A. and Bandyopadhyay, R., pages 91-100, chapter 5, International Atomic Energy Agency, ISBN 978-92-0-105110-3, 2010. |
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Relative resistance of some newly developed cassava cultivars to African cassava mosaic disease, Ariyo, O., Dixon, A. and Atiri, G.*, pages 541-545, International Society for Tropical Root Crops (ISTRC) - Africa Branch; Government of Benin, 2001. |
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Resistance of F1 segregating populations derived from crosses between wild banana accessions Musa acuminata spp. burmannicoides 'Calcutta 4' and M. balbisiana 'Montpellier' to black leaf streak disease, Vroh Bi, I., Zandjanakou, M, Mbah, W., Tenkouano, A., Ojiambo, P. and Bandyopadhyay, R., in: Acta Horticulturae, volume 828, pages 353-357, ISSN 0567-7572, 2009. |
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Resistance of tropical maize inbreds to major virus and virus-like diseases, Brewbaker, J., Kim, S. K. and Logrono, M.L., in: Maydica, volume 36, pages 257-265, 1991. |
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Resistance of water yam to anthracnose disease in Nigeria, Aduramigba-Modupe, A.O., Asiedu, R., Odebode, C. A.* and Oladiran, A., in: Proceedings of the African Crop Science Conference, 22-26 October 2001, Lagos, Nigeria, pages 483-486, African Crop Science Society, 2001. |
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Resistance profile of improved cassava germplasm to cassava mosaic disease in Nigeria, Egesi, C. N.*, Ogbe, F., Akoroda, M., Ilona, P. and Dixon, A., in: Euphytica, volume 155, pages 215-224, 2007. [DOI] |
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Screen house and field resistance of Taro cultivars to Taro Leaf Blight Disease (Phytophtora colocasiae), Fokunang, C. N.*, Mbong, G. A.*, Manju, E. B.*, Tembe, E.* and Hanna, R., in: British Biotechnology Journal, volume 15, number 1, pages 1-15, ISSN 2231-2927, 2016. [DOI] |
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Screening cassava cultivars for resistance to the cassava anthracnose disease (CAD), Ikotun, T.* and Hahn, S. K., in: Tropical root crops in a developing economy: proceedings of the 9th Symposium of the International Society for Tropical Root Crops, 20-26 October 1991, Accra, Ghana, pages 178-183, ISTRC; Government of Ghana; International Institue of Tropical Agriculture (IITA), 1994. |
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Screening landraces for additional sources of field resistance to Cassava Mosaic Disease and green mite for integration into the Cassava Improvement Program, Raji, A., Ladeinde, O.* and Dixon, A., in: Journal of Integrative Plant Biology, volume 50, number 3, pages 311-318, 2008. |
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Selection for resistance to cassava mosaic disease in African cassava germplasm using single nucleotide polymorphism markers, Codjia, E. D., Olasanmi, B.*, Agre, A. P., Uwugiaren, R., Ige, A. D. and Rabbi, I. Y., in: South African Journal of Science, volume 118, number 1-2: 11607, pages 1-7, ISSN 0038-2353, 2022. [DOI] |
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Serial analysis of gene expression (SAGE) of host-plant resistance to the cassava mosaic disease (CMD), Fregene, M.*, Matsumura, H., Akano, A., Dixon, A. and Terauchi, R., in: Plant Molecular Biology, volume 56, pages 563-571, 2004. |
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Silicon application enhances resistance to xanthomonas wilt disease in banana, Mburu, K., Oduor, R.*, Mgutu, A. J.* and Tripathi, L., in: Plant Pathology, pages 1-12, ISSN 0032-0862, 2015. [DOI] |
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Silicon, disease resistance, and yield of rice genotypes under upland cultural conditions, Winslow, M. D., in: Crop Science, volume 32, number 5, pages 1208-1213, ISSN 0011-183X, 1992. |
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Simple sequence repeats (SSR) diversity of cassava in South, East and Central Africa in relation to resistance to cassava brown streak disease, Pariyo, A., Tukamuhabwa, P.*, Baguma, Y.*, Kawuki, R. S.*, Alicai, T.*, Gibson, P. J.*, Kanju, E., Wanjala, B.*, Harvey, J., Nzuki, I., Rabbi, I. Y. and Ferguson, M., in: African Journal of Biotechnology, volume 12, number 28, pages 4453-4464, ISSN 1684-5315, 2013. [DOI] |
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Sources of resistance to cassava anthracnose disease, Owolade, F., Dixon, A., Adeoti, A.* and Osunlaja *, S., in: African Journal of Biotechnology, volume 4, number 6, pages 570-572, ISSN 1684-5315, 2005. |
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Sources of resistance to cowpea bacterial blight disease in Nigeria, Okechukwu, R. and Ekpo, E.*, in: Journal of Phytopathology, volume 152, pages 345-351, 2004. |
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Stability of resistance to cassava brown streak disease in major agro-ecologies of Uganda, Pariyo, A.*, Baguma, Y.*, Alicai, T.*, Kawuki, R. S.*, Kanju, E., Bua, A.*, Omongo, C. A.*, Gibson, P. J.*, Osiru, D.*, Mpairwe, D.* and Tukamuhabwa, P.*, in: Journal of Plant Breeding and Crop Science, volume 7, number 3, pages 67-78, ISSN 2006-9758, 2015. [DOI] |
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Strategies for controlling the Cassava Mosaic Disease Pandemic in Eastern and Central Africa through resistance breeding programs, Ntawuruhunga, P., Legg, J., Omongo, C. S.*, Lukombo, S.* and Kimani, E., 2006. |
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Strategies to resistance to bacterial wilt disease of banana through genetic engineering, Tripathi, L., Tripathi, J. and Tushemereirwe, W. K.*, in: African Journal of Biotechnology, volume 3, number 12, pages 688-692, ISSN 1684-5315, 2004. |
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Tackling the cassava mosaic disease (CMD) challenge in sub-Saharan Africa: the role of host-plabt resistance and germplasm deployment, Whyte, J., Mahungu, N. and Ng, S. Y. C., 2001. |
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Targeted knockout of early nodulin-like 3 (MusaENODL3) gene in banana reveals its function in resistance to Xanthomonas wilt disease, Ntui, V. O., Tripathi, J., Shah, T. and Tripathi, L., in: Plant Biotechnology Journal, volume 22, number 5, pages 1101-1112, ISSN 1467-7644, 2024. [DOI] |
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The genetics of resistance to cocoyam root rot blight complex disease in Xanthosoma sagittifolium(L.)Schott, Agueguia, A, Fatokun, C. and Hahn, S. K., in: Tropical root crops in a developing economy: proceedings of the 9th symposium of the International Society for Tropical RootCrops 20-26 October 1991, Accra Ghana, pages 438-442, ISTRC; Government of Ghana; International Institute of Tropical Agriculture (IITA), 1994. |
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The potential of hypersensitive response assisting protein (hrap) to enhance resistance against banana Xanthomonas wilt disease, Mwaka, H.*, Tripathi, L., Tripathi, J., Kiggundu, A.*, Arinaitwe, G.*, Tusiime, G.* and Tushemereirwe, W. K.*, in: Book of Abstracts in AGBIOSAFESEED 2010, March 8-11, Kampala, pages 52-53, 2010. |
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The relative resistance of cassava cultivars to African Cassava Mosaic Disease (ACMD) as determined by two methods: rank-sum and the area under the disease progress curve, Ariyo, O. and Atiri, G.*, in: Archives of Phytopathology and Plant Protection, volume 35, number 1, pages 23-30, 2002. |
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The use of biolistic inoculation of Cassava Mosaic begomoviruses in screening cassava for resistance to Cassava Mosaic Disease, Ariyo, O., Atiri, G.* and Winter, S., in: Journal of Virological Methods, volume 137, number 1, pages 43-50, 2006. |
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The use of bud grafting to evaluate cassava for resistance to African Cassava Mosaic Disease virus: poster presented at CBN-V, Nov. 4-9, 2001. DDPSC/ILTAB, St. Louis, Missouri, USA, Ogbe, F., Dixon, A., Atiri, G.* and Thottappilly, G., pages S8-14, 2001. |
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Training population optimization for prediction of Cassava Brown Streak Disease Resistance in west African clones, Ozimati, A.*, Kawuki, R. S.*, Ezuma, W.*, Kayondo, I. S.*, Wolfe, M., Lozano, R., Rabbi, I. Y., Kulakow, P. and Jannink, J., in: G3-Genes Genomes Genetics, volume 8, pages 3903-3913, ISSN 2160-1836, 2018. [DOI] |
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Transgenic banana expressing NH1 gene can provide resistance to bacterial and fungal diseases, Tripathi, J., Oduor, R.*, Ronald, P. C. and Tripathi, L., Abstract, p. 17 in 2016 World Congress on in Vitro Biology: meeting of the Society for in Vitro Biology June 11-16, San Diego, Califonia, 2016. |
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Transgenic banana expressing Pflp gene confers enhanced resistance to Xanthomonas wilt disease, Namukwaya, B., Tripathi, L., Tripathi, J., Arinaitwe, G.*, Mukasa, S. B. and Tushemereirwe, W. K.*, in: Transgenic Research, pages 1-11, ISSN 0962-8819, 2011. [DOI] |
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Transgenic bananas for disease resistance, Tripathi, L., Tripathi, J., Arinaitwe, G.*, Kiggundu, A.* and Tushemereirwe, W. K.*, Abstract (Oral Presentation) in the Book of Abstracts of the II ISHS Genetically Modified Organisms in Horticulture Symposium: Paving the Way for a Sustainable Future. Nelspruit, South Africa. September 12-16, 2011. |
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Transgenic bananas with enhanced resistance against Xanthomonas wilt disease, Tripathi, L., Tripathi, J., Tushemereirwe, W. K.*, Arinaitwe, G.* and Kiggundu, A.*, in: Acta Horticulturae, volume 974, pages 81-90, ISSN 0567-7572, 2013. |
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Transgenic technologies for developing bacterial disease resistance in plants, Tripathi, L., pages 200-220, Regency, 2005. |
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Two novel DNAs that enhance symptoms and overcome CMD2 resistance to cassava mosaic disease, Ndunguru, J.*, De Leon, L., Doyle, C. D., Sseruwagi, P.*, Plata, G., Legg, J., Thompson, G.*, Tohme, J., Aveling, T.*, Ascencio-Ibanez, J. T. and Hanley-Bowdoin, L., in: Journal of Virology, volume 90, number 8, pages 4160-4173, ISSN 0022-538X, 2016. [DOI] |
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Understanding the genetic basis of cassava mosaic disease resistance through in-silico mining and experimental gene discovery, Gedil, M., Rabbi, I. Y., Gisel, A. and Stavolone, L., Abstract in World Congress on Root & Tuber Crops (WCRTC), Nanning, Guangxi, China, January 18-22, 2016. |
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Unlocking Cassava Brown Streak Disease Resistance in Cassava: insights from genetic variability and combining ability, Sichalwe, K.*, Kayondo, S. I., Edema, R.*, Omari, M., Kulembeka, H.*, Rubaihayo, P.* and Kanju, E., in: Agronomy, volume 14, number 9: 14092122, pages 1-18, ISSN 2073-4395, 2025. [DOI] |
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Using CRISPR-Cas9 genome editing to enhance disease resistance in banana, Tripathi, J. and Tripathi, L., in: CABI Reviews, volume 19, number 1, pages 1-11, ISSN 1749-8848, 2024. [DOI] |
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Utility of Ugandan genomic selection cassava breeding populations for prediction of cassava viral disease resistance and yield in West African clones, Ozimati, A.*, Ezuma, W.*, Manze, F.*, Iragaba, P.*, Kanaabi, M.*, Ano, C. U.*, Egesi, C. and Kawuki, R. S.*, in: Frontiers in Plant Science, volume 13, number -: 1018156, pages 1-12, ISSN 1664-462X, 2022. [DOI] |
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Validation of diagnostic markers for streak virus disease resistance in maize, Sime, S. S., Menkir, A., Adetimirin, V.*, Gedil, M. and Kumar, P. L., in: Agriculture, volume 11, number 2: 130, pages 1-11, ISSN 2077-0472, 2021. [DOI] |
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Validation of KASP markers associated with cassava mosaic disease resistance, storage root dry matter and provitamin A carotenoid contents in Ugandan cassava germplasm, Ezuma, W.*, Eyoo, O.*, Gwandu, F.*, Mukasa, S.*, Alicai, T.*, Ozimati, A.*, Nuwamanya, E.*, Rabbi, I. Y. and Kawuki, R.*, in: Frontiers in Plant Science, volume 13, number : 1017275, pages 1-12, ISSN 1664-462X, 2022. [DOI] |
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Validation of single nucleotide polymorphism makers associated with cassava mosaic disease resistance in a clonal evaluation trial, Ishimwe, Y.*, Pan African University, 2019. |
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Verticillium dahliae disease resistance and the regulatory pathway for maturity and tuberization in potato, Tai, H. H., De Koeyer, D., Sonderkaer, M., Hedegaard, S., Lague, M., Goyer, C., Nolan, L., Davidson, C., Gardner, K., Neilson, J., Paudel, J. R., Murphy, A., Bizimungu, B., Wang, H. Y.*, Xiong, X.*, Halterman, D. and Nielsen, K. L., in: Plant Genome, pages 1-14, ISSN 1940-3372, 2017. [DOI] |
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Viral diseases of cowpea and their control by resistance-conferring genes, Hampton, R. O., Thottappilly, G. and Rossel, H. W., pages 159-175, chapter 14, International Institute of Tropical Agriculture (IITA); Japan International Research Center for Agricultural Sciences (JIRCAS), 1997. |
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Yield and disease resistance of plantain (Musa spp., AAB group) somaclones in Nigeria, Nwauzoma, A., Tenkouano, A., Crouch, J. H., Pillay, M., Vuylsteke, D. and Daniel-Kalio, L., in: Euphytica, volume 123, pages 323-331, 2002. |
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Comparison of field, greenhouse and detached-leaf evaluations of soybean germplasm for resistance to Phakopsora pachyrhizi, Twizeyimana, M., Ojiambo, P., Ikotun, T.*, Paul, C., Hartman, G. and Bandyopadhyay, R., in: Plant Disease, volume 91, number 9, pages 1161-1169, ISSN 0191-2917, 2007. |
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Keywords: | Disease resistance
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Fast-Track Release of Black Leaf Streak Resistant Banana and Plantain (Musa spp.) Hybrids and Related Technologies to Farmers in Cameroon: a Strategy to Enhance Food Security, Njukwe, E., Amah, D., Ndango, R. and Tenkouano, A.+, in: Proceedings of the International Conference on Banana and Plantain in Africa: Harnessing International Partnerships to Increase Research Impact, pages 577-584, ISHS, International Society for Horticultural Science, Belgium, 2010. |
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Keywords: | Banana and Plantain, Disease resistance, Fast-track release, Food securityye
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Cassava improvement in sub-Saharan Africa: contributions of IITA and its partners, Dixon, A., Okechukwu, R., Ssemakula, G., Hanna, R., Thresh, J. M., Hughes, J., Ingelbrecht, I., Fregene, M.*, Legg, J., Mahungu, N., Nweke, F., Ntawuruhunga, P., Fauquet, C., Manyong, V., Neuenschwander, P., Whyte, J., Wydra, K., Asiedu, R., Egesi, C. N.*, Bandyopadhyay, R., Winter, S., Tarawali, G., Bokanga, M., Ezedinma, C., Sanni, L., Ferguson, M., Ogbe, F., Akoroda, M., Maziya-Dixon, B., Ilona, P., Ekanayake, I. J., Ariyo, O., Onyeka, J.*, Otim-Nape, G.*, Dahniya, M. T., Ortiz, R., Hahn, S. K. and Hartmann, P., 2008. |
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Abstract: Cassava improvement in sub-Saharan Africa: contributions of IITA and its partners
Dixon, A.G.O., R.U. Okechukwu, G. Ssemakula, R. Hanna, J.M. Thresh, J.d'A. Hughes, I. Ingelbrecht, M. Fregene, J. Legg, N. Mahungu, F. Nweke, J.P. Legg, P. Ntawuruhunga, C. Fauquet, V. Manyong, P. Neuenschwander, J. Whyte, K. Wydra, R. Asiedu, C.N. Egesi, R. Bandyopadhyay, S. Winter, G. Tarawali, M. Bokanga, C. Ezedinma, L. Sanni, M. Ferguson, F.O. Ogbe, M. Akoroda, B. Maziya-Dixon, P. Ilona, I.J. Ekanayake, O. Ariyo, J. Onyeka, G.W. Otim-Nape, M.T. Dahniya, R. Ortiz, S.K. Hahn and P. Hartmann
Cassava is well recognized for its capacity to address food needs of vulnerable communities in unstable environments in SSA. IITA and colleagues in African NARS, in collaboration with CIAT and ARIs have played leading roles in the development of improved cassava varieties which are disease and pest resistant, early maturing, and high yielding. Through a combination of conventional and new approaches, over 400 cassava genotypes have been developed. The characteristics of the new generation of cassava germplasm broke what had been an apparent yield barrier in cassava improvement increasing yields in many locations by at least 50-100% without the use of fertilizer. The improved germplasm is shared with NARS within the region as specific genotypes or improved seed populations for evaluation and selection under local conditions. Improvement programs in Africa that received these materials have tested them under local conditions, selected varieties that outperform local varieties, and released them to farmers in virtually every major cassava producing country. Today, about 30% of the area cropped with cassava in Africa is planted with improved varieties. Without the introduction of more productive cultivars with multiple diseases and pest resistance, the effective biological control of the cassava mealybug and, to a certain extent, of the green mite, cassava production in SSA would be 50% or less of what it is today. That translates to over 13 million tons of dry cassava/year, enough to meet the calorie requirements of 65 million people. The significant gains in the crop's output in farmers' fields are not only contributing to the African diet but also propelling commercialization of the crop. This paper highlights contributions to cassava improvement in SSA since 1970 by IITA and its partners, and suggests areas needing strengthening in the drive to produce better crop varieties for different regions and enduses in Africa.
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Characterisation of resistance in cassava against viruses causing cassava mosaic and cassava brown streak disease in Africa, Koerbler, M., Stein, B., Ingelbrecht, I., Dixon, A. and Winter, S., 2008. |
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Abstract: Cassava mosaic disease caused by diverse geminiviruses (CMG) and Cassava brown streak disease caused by cassava brown streak virus (CBSV), are key constraints to the cultivation of cassava in Sub-Sahara Africa. We have collected and characterised the diversity of the most prominent geminiviruses from all African cassava cultivation areas and Cassava brown streak viruses from East Africa, to use defined viruses for resistance studies in selected IITA cassava breeding lines and African land races. Virus infections were established in cassava by either graft inoculation with scions of virus infected plants or, by particle bombardment of cloned infectious viruses. Resistance against cassava mosaic geminiviruses was identified in several breeding lines e.g. TME 4, TMS 96/0529 and TMS 96/0160 responding with abortion of virus infections after virus introduction. Other genotypes, e.g. TMS 96/0304, became infected but recovered from symptoms but maintaining the infection status. When cassava clones were analysed for CBSV resistance, it became evident that geminivirus resistance was tightly correlated with susceptibility against CBSV. Geminivirus resistant cassava responded with often severe CBSV infections (e.g. TMS 96/0160) upon virus inoculation. Interestingly, cassava genotypes with an intermediate resistance, TMS 96/0304, initially became mixed infected with CBSV/CMG however subsequently aborting CMG, to establish single CBSV infections. Differential responses were also recorded for strains of CBSV. While CMG resistance in cassava was against all CMG, differential responses were observed in cassava inoculated with CBSV isolates obtained from Kenya and Mozambique. While the CMG susceptible cassava landrace TME 117 was resistant against CBSV from Kenya, it became infected with CBSV from Mozambique. Only the cassava genotype TMS/ 1089A revealed immunity against CMG and also did not establish infections with the CBSV isolates from diverse origins.
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Evaluation of water yam (Dioscorea alata L.) germplasm for reaction to yam anthracnose and virus diseases and their effect on yield, Egesi, C. N.*, Odu, B., Ogunyemi, S., Asiedu, R. and Hughes, J., in: Journal of Phytopathology, volume 155, number 9, pages 536-543, 2007. |
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Abstract: Use of genetic resistance is the most practical and economic way to manage major diseases of yams. In a search for sources of resistance, 40 water yam (Dioscorea alata L.) accessions from Benin, Ghana, Nigeria and Puerto Rico were screened under natural disease infection conditions in Ibadan, Nigeria. The accessions were evaluated at 1, 3 and 6 months after planting (MAP) for severity of yam anthracnose and viral diseases. The effect of the pathogens on yield was also evaluated at harvest 9 MAP. There were significant differences (P < 0.001) between accessions for severities of anthracnose and viral diseases. Eight (20%) of them had lower anthracnose area under disease progress curves (AUDPC) values than the resistant check while 10 (25%) had AUDPC values below the trial mean. There were significant variations (P < 0.001) in yield components among the accessions. There was significant negative correlation of anthracnose severity with fresh tuber yield (r = -0.51) and with number of tubers per plot (r = -0.40). Similarly, significant negative correlations were observed of virus disease severity with fresh tuber yield (r = -0.78) and number of tubers per plot (r = -0.65). Linear regression models also showed that the fresh yield had significant negative relationships with anthracnose (R2 = 0.26) and viral (R2 = 0.62) diseases. The accessions identified as resistant constitute a valuable resource for breeding of resistant germplasm.
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Genetic gains from 30 years of cassava breeding in Nigeria for storage root yield and disease resistance in elite cassava genotypes, Okechukwu, R. and Dixon, A., in: Journal of Crop Improvement, volume 22, number 2, pages 181-208, 2008. |
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Abstract: Cassava (Manihot esculenta Crantz) is crucial for both food security and poverty alleviation in Sub-Saharan Africa (SSA). Cassava improvement for SSA started at the International Institute of Tropical Agriculture in 1970, and several improved lines with different characteristics have been developed to date. The primary focus of breeding work has been to increase root yield, early bulking, tolerance to biotic and abiotic stresses, and increased dry matter content. This paper represents a study of 112 varieties cloned and introduced between 1970 and 2000, and
evaluated in 2003 and 2004 to quantify genetic gains in root yield and disease resistance. The genetic gain per year was 1.3% for fresh root yield, 1.2% for dry root yield, 0.65% for cassava mosaic disease resistance, 0.21% for cassava anthracnose disease resistance, and -0.03% for cassava bacterial blight disease resistance. Though there was no statistical significance in the net negative genetic gain in cassava bacterial blight resistance, it is essential that more emphasis be placed on improving this trait to enhance stability and productivity in African environments.
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Novel sources of resistance to Fusarium stalk rot of maize in tropical Africa, Afolabi, C., Ojiambo, P., Ekpo, E.*, Menkir, A. and Bandyopadhyay, R., in: Plant Disease, volume 92, number 5, pages 772-780, ISSN 0191-2917, 2008. |
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Abstract: Fusarium stalk rot is one of the most widespread and destructive diseases of maize, and deployment of resistant genotypes is one of the most effective strategies for controlling the disease. Fifty inbred lines and four checks from the breeding program of the International Institute of Tropical Agriculture were evaluated in field trials at Ikenne and Ibadan, Nigeria in 2003 and 2004 to identify new sources of resistance to stalk rot caused by Fusarium verticillioides. Evaluations were conducted under artificial inoculation and natural infection at Ibadan and Ikenne, respectively. Disease severity was recorded using a severity scale (SS) and direct estimation of stalk discoloration (SD). The two methods of disease assessment were compared and combined to classify genotypes into resistance groups using results from rank-sum analysis. In 2003, disease severity ranged from SS = 1 to 5 and SD = 1.3 to 33.8% at both locations. Both SS and SD were significantly (P < 0.01) higher in 2003 than in 2004 at the two locations. In both years, inbred lines significantly differed in SS (P < 0.02) and SD (P < 0.04) at Ibadan. Similarly, inbred lines significantly differed in SS (P < 0.04) and SD (P < 0.04) when genotypes were evaluated at Ikenne. Disease assessments based on SS and SD were significantly correlated (0.68 < r < 0.95, P < 0.01) in both years. Based on the results from rank-sum analysis, inbred lines were separated into highly resistant, resistant, moderately resistant, moderately susceptible, susceptible, and highly susceptible groups. At Ibadan, 6 (11.1%) and 8 (14.8%) were identified as highly resistant and resistant, respectively, whereas 11 (20.4%) were identified as resistant at Ikenne. Inbred lines 02C14609, 02C14643, 02C14654, and 02C14678 were consistently classified as either highly resistant or resistant to stalk rot across locations and years while the check genotypes were classified either as susceptible or moderately susceptible to stalk rot. These four inbred lines identified to have high levels of disease resistance may be used for breeding maize with resistance to Fusarium stalk rot.
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Screening landraces for additional sources of field resistance to Cassava Mosaic Disease and green mite for integration into the Cassava Improvement Program, Raji, A., Ladeinde, O.* and Dixon, A., in: Journal of Integrative Plant Biology, volume 50, number 3, pages 311-318, 2008. |
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Abstract: Twelve cassava landraces were evaluated for sources of resistance genes to diseases and pests of major economic importance in Africa. The objective was to assess their levels of field resistance to mosaic disease (ACMD), bacterial blight (CBB), anthracnose (CAD), and green mite (CGM), compared to TMS30572, an elite cultivar widely adopted in Africa. Considerable genotypic variation was observed among cultivars for resistance to ACMD and CGM but not for CBB and CAD. The lowest mean incidence of 12% and severity of 1.8 on a scale of 1-5 for ACMD was recorded for Atu, a landrace with farmer acceptable qualities. In comparison, the improved cultivar, TMS 30572, had a mean disease incidence of 72% and a severity score of 2.8. Another landrace, MS-20 had the lowest CGM damage score (2.1) while TMS 30572 emerged as one of the susceptible cultivars with a damage score of 3. Additional sources of resistance to ACMD and CGM that may possibly be better than the popular improved cultivar, TMS 30572, were identified in this study. These could serve as novel sources of additional genes to complement existing resources for elite cassava breeding in Africa.
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Severity of anthracnose and virus diseases of water yam (dioscorea alata L.) in NIgeria I: effects of yam genotype and date of planting, Egesi, C. N.*, Onyeka, T. and Asiedu, R., in: Crop Protection, volume 26, number 8, pages 1259-1265, ISSN 0261-2194, 2007. |
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Abstract: Anthracnose and virus diseases exert devastating impacts on yam production in many tropical regions of the world where the crop contributes to food security and income generation. The complexities in their epidemiology necessitate the use of integrated approaches in their management. Six genotypes of Dioscorea alata, planted on six dates (March?August) in each of 1998 and 1999 in Ibadan, Nigeria, were evaluated to determine the effects of planting date and genotype on severity of the two diseases. The date of planting had a profound influence on anthracnose severity, contributing 72% of the observed variation due to combined effects of planting date, genotype and their interactions. In contrast, the genotype effect was most dominant with respect to virus severity, accounting for 85% of the total observed variation. Planting in August supported least anthracnose development while April and May plantings resulted in the least severity of virus diseases. While selection of planting time could be used to manage anthracnose disease, its application could be influenced by the prevailing weather conditions in a particular location. Two of the genotypes (TDa 291 and TDa 297) had low severity values for both diseases across the different planting dates. Availability of such sources of multiple disease resistance and appropriate choice of planting date would be very useful in integrated management of the two major yam diseases.
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Somatic embryogenesis and genetic transformation of African farmer-grown cassava genotypes that are susceptible to the Cassava Brown Streak Disease, Ingelbrecht, I., Raji, A., Oyelakin, O., Winter, S., Moller, B., Dixon, A. and Jorgensen, K., 2008. |
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Abstract: Cassava is one of the most important sources of carbohydrates for over 500 million people in the (sub)tropics. Cassava plays an important role in the food security of many developing nations, especially in sub-Saharan Africa (SSA). Cassava is highly heterozygous, genetically complex, and many varieties either do not flower or produce few seeds thus hampering conventional crop improvement. Several major constraints limit the production and utilization of cassava roots, including two viral diseases, the Cassava Brown Streak Disease (CBSD) and the Cassava Mosaic Disease (CMD), which are specific to SSA. Farmer-preferred landraces are often susceptible to CBSD and/or CMD. Genetic transformation of cassava could complement conventional breeding programs for CBSD and/or CMD resistance. Current protocols for genetic transformation of cassava are limited to model genotypes which are not used by farmers of breeders in SSA. Since transformation protocols are genotype-dependent, suitable procedures for genetic modification of the landraces need to be developed. We have established somatic embryogenesis and organogenesis for three farmer/breeder-preferred varieties, two from East Africa (cv Kibaha and cv Albert) and one from West Africa (TME12) which are susceptible to CBSD. Primary and cyclical somatic embryogenesis was established for the three varieties. Cotyledon tissues from somatic embryos were used as source explants for Agrobacterium-mediated genetic transformation. Using an intron-interrupted {\^a}{\"i}¿½glucuronidase reporter gene construct under control of the Cassava vein mosaic virus promoter, stably transformed cassava tissues and plants were obtained. Molecular evidence for stable expression of the transgenes will be presented.
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