| Assessment of biochemical, cooking, sensory and textural properties of the boiled food product of white yam (D. rotundata) genotypes grown at different locations, Alamu, E. O., Adesokan, M., Awoyale, W., Oyedele, H. A., Fawole, S., Asfaw, A. and Maziya-Dixon, B., in: Heliyon, volume 8, number 12: e11690, pages 1-7, ISSN 2405-8440, 2022. [DOI] |
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| Comparative effects of explant source and genotype on in vitro tuberization in Dioscorea alata and D. rotundata, Balogun, M., Ng, S. Y. C., Shiwachi, H ., Ng, N. Q. and Fawole, I., in: Tropical Science, volume 44, number 4, pages 196-200, 2004. |
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| Development of mapping populations for genetic analysis in yams (D. rotundata and D. alata), Sartie, A. M. and Asiedu, R., in: Tropical Roots and Tuber Crops and the Challenges of Globalization and Climate Changes. Proceedings of the 11th triennial Symposium of the ISTRC-AB, 4 to 8 October 2010, pages 101-111, ISTRC-AB, Ibadan, Nigeria, 2012. |
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| Development of mapping populations for genetic analysis in yams (Dioscorea rotundata and D. alata), Sartie, A. M. and Asiedu, R., Abstract (Oral presentation) in Book of Abstracts of the 11th Triennial Symposium of ISTRC-AB, held at Memling Hotel, Kinshasa. 4-8 October, 2010. |
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| Domestication and evolutionary dynamics of cultivated yam (Dioscorea cayenensis /D. rotundata complex) in Benin, Dansi, A.*, Adjatin, A.*, Bhattacharjee, R., Asiedu, R., Vodouhe, R., Akouegninou, A.* and Sanni, A.*, Abstract in First Global Conference on Yam 'to harness research innovations to unleash the potential of yam', 3-6 October, 2013 Accra, Ghana, 2013. |
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| Effects of basal medium and plant growth regulator regimes on meristem and nodal cultures in white yam (D. rotundata), Ajayi, A., Balogun, M., Maroya, N. and Asiedu, R., YIIFSWA Research Brief, 2017. |
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| Genetic diversity and relationship of Guinea yam (Dioscorea cayenensis Lam.-D. rotundata Poir. complex) germplasm in Benin (West Africa) using microsatellite markers, Loko, Y.*, Bhattacharjee, R., Agre, A. P.*, Dossou-Aminon, I.*, Orobiyi, A.*, Djedatin, G. L.* and Dansi, A.*, in: Genetic Resources and Crop Evolution, pages 1-15, ISSN 0925-9864, 2016. [DOI] |
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| Identification of candidate flowering and sex genes in white Guinea yam (D. rotundata Poir.) by SuperSAGE transcriptome profiling, Girma Tessema, G., Natsume, S., Carluccio, A., Takagi, Hiroki, Matsumura, H., Uemura, A., Muranaka, S., Takagi, Hiroko, Stavolone, L., Gedil, M., Spillane, C., Terauchi, R. and Tamiru, M., in: PloS ONE, volume 14, number 9: e0216912, pages 1-18, ISSN 1932-6203, 2019. [DOI] |
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| Influence of explant condition on microtuberization of yams (Dioscorea alata and D. rotundata), Kikuno, H ., Shiwachi, H ., Onjo, M. and Asiedu, R., in: Japanese Society for Tropical Agriculture, volume 48, number 2, pages 71-72, 2004. |
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| Introduction and evaluation of improved varieties of Dioscorea rotundata, and D. cayenensis with high micronutrient content in Cameroon, Njukwe, E., Amah, D., Ndango, R., Tenkouano, A. and Asiedu, R., in: Root and tuber crops for poverty alleviation through science and technology for sustainable development: proceedings of the 10th symposium of ISTRC-AB held from 8-12 October, 2007 in Maputo, Mozambique, pages 338-342, ISTRC-AB, 2010. |
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| Mini tuber production of white yam (D. rotundata) using vine cutting, Kikuno, H ., Muamba, K., Shiwachi, H ., Onjo, M. and Asiedu, R., in: 99th meeting of the Japanese Society Tropical Agriculture, Tsukuba, Japan, pages 41-42, 2006. |
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| Morphological diversity, cultivar groups and possible descent in the cultivated yams (Dioscorea cayenensis/D. rotundata) complex in Benin Republic, Dansi, A., Mignouna, H., Zoundjikekpon, J., Sangare, A.*, Asiedu, R. and Quin, F., in: Genetic Resources and Crop Evolution, volume 46, number 4, pages 371-388, ISSN 0925-9864, 1999. |
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| Ploidy level of the cultivated yams (Dioscorea cayenensis/D. rotundata complex) from Benin Republic as determined by chromosome counting and flow cytometry, Dansi, A., Pillay, M., Mignouna, H., Dainou, O., Mondeil, F. and Moutairou, K.*, in: African Crop Science Journal, volume 8, number 4, pages 355-364, ISSN 1021-9730, 2000. |
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| Retention of iron and zinc in yam flour and boiled yam processed from white yam (D. rotundata) varieties, Maziya-Dixon, B., Alamu, E. O., Wireko-Manu, F. D.* and Asiedu, R., in: Food Science & Nutrition, volume 5, number 3, pages 662-668, ISSN 2048-7177, 2017. [DOI] |
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| SSR markers reveal diversity in Guinea yam (Dioscorea cayenensis/D. rotundata) core set, Obidiegwu, J., Kolesnikova-Allen, M.+, Ene-obong, E. E.*, Muoneke, C. O.* and Asiedu, R., in: African Journal of Biotechnology, volume 8, number 12, pages 2730-2739, ISSN 1684-5315, 2009. |
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| The possibility of vine cutting techniques on white yam (D. rotundata) using natural compounds, Kikuno, H ., Ayankanmi, T., Shiwachi, H . and Asiedu, R., in: 99th Meeting of the Japanese Society Tropical Agriculture, Tsukuba, Japan, pages 43-44, 2006. |
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| Viruses infecting cultivated yams (Dioscorea alata and D. rotundata) in Nigeria, Hughes, J., Dongo, L. and Atiri, G.*, in: Phytopathology, volume 87, number S-45, 1997. |
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| Yam (Dioscorea rotundata Poir. and D. alata L.) meristem culture - optimization and genetic stability study of micropropagated plants, Adeniyi, O. J., Adetimirin, V.*, Ingelbrecht, I. and Asiedu, R., in: 9th Triennial Symposium of the International Society for Tropical Root Crops-Africa Branch (ISTRC-AB); October 31 to November 5, 2004, Mombasa, Kenya, 2004. |
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| GGE biplot analysis of Dioscorea rotundata cultivar tela genotypes in Ghana, Otoo, E.* and Asiedu, R., in: Journal of Food, Agriculture and Environment, volume 7, number 1, pages 134-139, ISSN 1459-0255, 2009. |
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| Keywords: | D. rotundata; agroecologies; genotypes; genotype x environment interaction
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| The performance profile of Dioscorea rotundata cultivar Dorban genotypes in Ghana using GGE biplot analysis, Otoo, E.* and Asiedu, R., in: Journal of Food, Agriculture and Environment, volume 7, number 1, pages 150-155, ISSN 1459-0255, 2009. |
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| Keywords: | D. rotundata; genotypes; genotype x environment interaction
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| First report of cucumber mosaic virus in yams (Dioscorea spp.) in Ghana, Togo and Republic of Benin in West Africa, Eni, A., Kumar, P. L., Asiedu, R., Alabi, O., Naidu, R.*, Hughes, J. and Rey, M.*, in: Plant Disease, volume 92, number 5, pages 833, ISSN 0191-2917, 2008. [DOI] |
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Abstract:
Yam (Dioscorea spp., family Dioscoreaceae) is one of the most important food crops cultivated in the West African yam zone comprising the forest and savannah areas of Nigeria, Ghana, Cote d'Ivoire, Republic of Benin, and Togo, which account for more than 90% of the 4.59 million ha of yam cultivation worldwide (1). A survey was conducted in 2005 to document viruses in yams in Ghana, Togo, and the Republic of Benin. Samples (1,405) from five species of yam showing mosaic, chlorosis, and stunting as well
as asymptomatic plants were tested for Dioscorea bacilliform virus (DBV, genus Badnavirus), Yam mosaic virus (YMV, genus Potyvirus), and Yam mild mosaic virus (YMMV, genus Potyvirus), the three most common viruses infecting yams. In addition, samples were tested for Cucumber mosaic virus (CMV), since CMV was previously reported to infect yams in Cote d'Ivoire (2) and Nigeria (3). In protein-A sandwich-ELISA with polyclonal antibodies to a cowpea isolate of CMV, 23 of the 1,405 samples (6 of 218 samples from Togo, 13 of 628 samples from Ghana, and 4 of 559 samples from Republic of Benin) tested positive for CMV.
The CMV-positive samples were from D. alata (N = 16) and D. rotundata (N = 7), whereas all samples from D. cayenensis, D. dumetorum, and D. bulbifera tested negative. CMV was detected as mixed infections with DBV, YMV, or YMMV in 21 of 23 samples. Some of these samples showed puckering, chlorosis, mottling, and crinkling, whereas some plants infected by two or more viruses were asymptomatic. Only two samples from D. rotundata had a single infection of CMV and they showed mild chlorotic symptoms in young leaves that were inconspicuous in mature leaves. In sap inoculations, the virus induced systemic mosaic in
Nicotiana glutinosa. The presence of CMV in ELISA-positive yam samples was further confirmed by immunocapture-reverse transcription (IC-RT)-PCR using CMV antibodies as trapping antibody and oligonucleotide primers specific for a 485 nt corresponding to 3' end of the coat protein gene and C-terminal noncoding region of RNA-3 (4). To confirm the specificity of ICRT-PCR, the 485-bp amplicons from an isolate from the Republic of Benin was cloned into pCR2.1 (Invitrogen, Carlsbad, CA) and three independent clones were sequenced from both orientations. Pairwise comparison of a consensus sequence (Accession No. EU274471) with corresponding sequences of other CMV isolates deposited in GenBank showed 99% identity at the nucleotide sequence level (Accession No. U22821) and revealed that the CMV isolate from yam belongs to sub-Group IA. To our knowledge,
this is the first report of CMV infection in yams (D. alata and D. rotundata) in Ghana, Togo, and the Republic of Benin. Together with a previous documentation of CMV in D. alata and D. trifida in Cote d'Ivoire and Nigeria (2,3), this report adds to existing knowledge on distribution of CMV in yams with implications for yam production and germplasm distribution in the West Africa Region.
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