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Cultural control of scutellonema bradys on Dioscorea spp., Claudius-Cole, A. O., Asiedu, R. and Fawole, B., in: Root crops: the small processor and development of local food industries for market economy: proceeding of the Eighth Triennial Syposium of the International Society for Tropical Root Crops - Africa Branch (ISTRC-AB), 12-16 November 2001, Ibadan, Nigeria, pages 572-576, 2003.
Culturing meristematic tissue and node cuttings from yams (Dioscorea), Mignouna, H. D.*, Abang, M. M., Asiedu, R. and Geeta, R., in: Cold Spring Harbor Protocols, volume 4, number 11, ISSN 1559-6095, 2009. [DOI]
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Studies on the antioxidant and whitening properties of extracts from tuber of yams (Dioscorea spp.), Park, B., Kikuno, H ., Asiedu, R. and Park, C. H., in: Proceedings of Japanese Society for Tropical Agriculture: Research for Tropical Agriculture, 18-19 October, 2008, pages 47-48, 2008.
Studies on the reproductive biology of white yam (Dioscorea rotundata Poir.), Segnou, , Fatokun, C., Akoroda, M. and Hahn, S. K., in: Euphytica, volume 64, pages 197-203, ISSN 0014-2336, 1992.
Suitability of Dioscorea alata genotypes for making traditional food products in West Africa, Baah, F., Maziya-Dixon, B., Asiedu, R., Ellis, W.* and Oduro, I.*, in: Fourteenth triennial symposium of ISTRC, Trivandrum, Kerala, India, 2006, pages 36, International Society for Tropical Root Crops (ISTRC), 2006.
Survey of Scutellonema bradys incidence on marketed yam (Dioscorea spp.) in Benin, Baimey, H., Coyne, D. and Labuschagne, N.*, in: African Journal of Root and Tuber Crops, volume 7, number 1, pages 8-16, ISSN 1118-2075, 2009.
Survey of the incidence and distribution of viruses infecting yam (Dioscorea spp.) in Ghana and Togo, Eni, A, Hughes, J.+, Asiedu, R. and Rey, M., in: Annals of Applied Biology, volume 156, number 2, pages 243-251, ISSN 0003-4746, 2010.
Targetted genome editing in yam (dioscorea spp.) using the crispr/Cas9 system, David, E. S.*, University of Nairobi, 2021.
The Improvement of Yam (Dioscorea spp. through Breeding, Wilson, J. E., 1978.
The influence of arbuscular mycorrhizal fungi inoculation on micro-propagated hybrid yam (Dioscorea spp.) growth and root knot nematode (Meloidogyne spp.) suppression, Tchabi, A., Hountondji, F., Ogunsola, B., Lawouin, L., Coyne, D., Wiemken, A. and Oehl, F., in: International Journal of Current Microbiology and Applied Sciences, volume 5, number 10, pages 267-281, ISSN 2319-7692, 2016. [DOI]
The influence of minisett size and time of planting on the yield of seed yam (Dioscorea Rotundata), Aighewi, B., Maroya, N., Mignouna, D., Aihebhoria, D., Balogun, M. and Asiedu, R., in: Experimental Agriculture, volume 56, number 3, pages 469-481, ISSN 0014-4797, 2020. [DOI]
The performance profile of Dioscorea rotundata cultivar Dorban genotypes in Ghana using GGE biplot analysis, Otoo, E.* and Asiedu, R., in: Journal of Food, Agriculture and Environment, volume 7, number 1, pages 150-155, ISSN 1459-0255, 2009.
The prevalence of badnaviruses in West African yams (Dioscorea cayenensis-rotundata) and evidence of endogenous pararetrovirus sequences in their genomes, Seal, S., Turaki, A., Muller, E., Kumar, P. L., Kenyon, L., Filloux, D., Galzi, S., Lopez-Montes, A. and Iskra-Caruana, M. L., in: Virus Research, volume 186, pages 144-154, ISSN 0168-1702, 2014. [DOI]
The relevance of stomatal frequency, type and size in the delimitation of three complex species of Dioscorea L. (Dioscoreaceae), Ugborogho, R. E., Ng, N. Q., Thottappilly, G. and Hahn, S. K., in: Feddes Repertorium, volume 104, number 7-8, pages 485-496, 1993.
The susceptibility of the root-knot nematode, Meloidogyne incognita, Race 2, on yams, Dioscorea spp.: paper presented at the 8th Annual Conference of the Nigerian Society for Plant Protection, Benin City, February 27-March 1, 1978, Caveness, F. E., 1978.
Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses, Bomer, M., Rathnayake, A. I., Visendi, P., Sewe, S. O., Sicat, J. P. A., Silva, G., Kumar, P. L. and Seal, S., in: Physiological and Molecular Plant Pathology, pages 1-13, ISSN 0885-5765, 2018. [DOI]
Towards genetic improvement of yams (Dioscorea spp.) in Africa, Asiedu, R., in: Tropical Root and Tuber Crops Bulletin, volume 8, number 1, pages 8-9, 1994.
True yams (Dioscorea): a biological and evolutionary link between eudicots and grasses, Mignouna, H. D.*, Abang, M. M., Asiedu, R. and Geeta, R., in: Cold Spring Harbor Protocols, volume 4, number 11, ISSN 1559-6095, 2009. [DOI]
Two genetically distinct populations of colletotrichum gloeosporioides from yam (Dioscorea spp.) in Nigeria, Abang, M., Fagbola, O.*, Smalla, K., Asiedu, R. and Winter, S., in: 9th Triennial Symposium of the Internaitonal Society for Tropical Root Crops-Africa Branch (ISTRC-AB); October 31 to November 5, 2004, Mombasa, Kenya, 2004.
Understanding the genetic diversity and population structure of yam (Dioscorea alata L.) using microsatellite markers, Arnau, G., Bhattacharjee, R., Sheela, M.N., Chair, H., Malapa, R., Lebot, V., Abraham, K.*, Perrier, X., Petro, D., Penet, L. and Pavis, C., in: PLoS ONE, volume 12, number 3(e0174150), pages 1-17, ISSN 1932-6203, 2017. [DOI]
Using isozyme polymorphism to assess genetic variation within cultivated yams (Dioscorea cayenensis/Dioscorea rotundata complex) of the Republic of Benin, Dansi, A., Mignouna, H., Zoundjihekpon, J., Sangare, A.*, Asiedu, R. and Ahoussou, N., in: Genetic Resources and Crop Evolution, volume 47, pages 371-383, 2000.
Variation in nutrient and antinutrient contents of tubers from yellow yam (Dioscorea cayenensis) genotypes grown at two locations, Akin-Idowu, P., Odunola, O., Asiedu, R., Maziya-Dixon, B. and Uwaifo, A. O., in: Journal of Food, Agriculture and Environment, volume 6, number 3-4, pages 95-100, ISSN 1459-0255, 2008.
Variation in pasting and functional properties of flours and starches from water yam (Dioscorea alata), Oke, M. O., Awonorin, S., Asiedu, R., Maziya-Dixon, B. and Sanni, L., in: Securing livelihoods through yams: proceedings of a technical workshop on progress in yam research for development in west and central Africa held in Accra, Ghana, 11-13 September, 2007, pages 269-278, International Institute of Tropical Agriculture (IITA), 2009.
Variation in tuber dry matter content and starch pasting properties of white Guinea yam (Dioscorea rotundata) genotypes grown in three groecologies of NIGERIA, Matsumoto, R., Asfaw, A., De Koeyer, D., Muranaka, S., Yoshihashi, T., Ishikawa, H., Adebola, P. O. and Asiedu, R., in: Agronomy, volume 11, number 10: 1944, pages 1-15, ISSN 2073-4395, 2021. [DOI]
Varietal identification key of the cultivated yams (Dioscorea cayenensis / Dioscorea rotundata complex) of Benin republic, Dansi, A., Mignouna, H., Zoundjihekpon, J., Sangare, A.* and Asiedu, R., in: Plant Genetic Resources Newsletter, volume 116, pages 18-25, 1998.
Vegetative Compatibility Among Isolates of Colletotrichum gloeosporioides from Yam (Dioscorea spp.) in Nigeria, 2004.
Vegetative compatibility among isolates of Colletotrichum gloeosporioides from yam (Dioscorea spp.) in Nigeria, Abang, M., Hoffmann, P., Winter, S., Green, K. and Wolf, G., in: Journal of Phytopathology, volume 152, pages 21-27, ISSN 0931-1785, 2004.
Viruses in weeds in Dioscorea yam fields in Nigeria, Asala, S., Alegbejo, M., Kashina, B. D., Banwo, O. O.* and Shinggu, C. P.*, in: African Crop Science Journal, volume 22, number 2, pages 109-115, ISSN 1021-9730, 2014.
Viruses in weeds in Dioscorea yam fields in Nigeria, Asala, S., Shinggu, C. P., Asiedu, R. and Kumar, P. L., Abstract, 2010.
Viruses infecting cultivated yams (Dioscorea alata and D. rotundata) in Nigeria, Hughes, J., Dongo, L. and Atiri, G.*, in: Phytopathology, volume 87, number S-45, 1997.
Virus-like Particles and Cytoplasmic Inclusions Associated with Disease Dioscorea rotundata Poir from Nigeria, Mohammed, N. and Terry, E. R., 1978.
Virus-like particles and cytoplasmic inclusions associated with diseased Dioscorea rotundata poir from Nigeria, Mohamed, N. A. and Terry, E. R., in: Tropical Agriculture (Trinidad), volume 56, number 2, pages 175-179, ISSN 0041-3216, 1979.
Water yam (Dioscorea alata L.) growth and tuber yield as affected by rotation and fertilization regimes across an environmental gradient in west Africa, Pouya, N., Hgaza, V. K., Kiba, D. I., Bomisso, L., Aighewi, B., Ake, S. and Frossard, E., in: Agronomy, volume 12, number 4: 792, pages 1-22, ISSN 2073-4395, 2022. [DOI]
Water yam (Dioscorea alata), a neglected crop with high potential in West Africa, Okonkwo, C., Adeniji, M. and Asiedu, R., in: 9th Triennial Symposium of International Society for Tropical Root Crops-Africa Branch (ISTRC-AB); October 31 to November 5, 2004, Mombasa, Kenya, 2004.
Weed interference and control in white yam (Dioscorea rotundata Poir), Akobundu, I., in: Weed Research, volume 21, pages 267-272, ISSN 0043-1737, 1981. [DOI]
Wet-cold preservation of West African yam (Dioscorea spp.) pollen, Daniel, I., Ng, N. Q., Tayo, T. and Togun, A., in: Journal of Agricultural Science, volume 138, pages 57-62, 2002.
White Guinea yam (Dioscorea rotundata Poir.) landraces trait profiling and setting benchmark for breeding programs in the Republic of Benin, Loko, Y., Gbemavo, C., Agre, A. P., Dassou, A. G., Yelome, O. I., Idossou, R., Afoha, S. A. P., Dadonougbo, E., Fakorede, J. and Dansi, A.*, in: PloS ONE, volume 17, number 8: e0273043, pages 1-22, ISSN 1932-6203, 2022. [DOI]
Whole genome sequencing of White guinea yam (Dioscorea rotundata): towards generating genetic and genomic tools for improvement of an African 'orphan' crop, Oli, M. T., Natsume, S., Takagi, H., Yoshida, K., Yaegashi, H., Uemura, A., Oikawa, K., Uraski, N., Matsumura, H., Babil, P., Yamanaka, S., Matsumoto, R., Muranaka, S., Girma Tessema, G., Lopez-Montes, A., Gedil, M., Bhattacharjee, R., Abberton, M., Kumar, P. L., Rabbi, I. Y., Kahl, G., Takagi, H., Asiedu, R. and Terauchi, R., Abstract, p. w899, in the book of abstracts of the Plant and Animal Genome XXIV Conference January 08 - 13, San Diego, CA, 2016.
Whole-genome sequencing-based analysis of diversity in Guinea yam (Dioscorea rotundata) breeding materials: a preliminary result, Tamiru, M., Natsume, S., Takagi, Hiroki, Babil, P. K., Yamanaka, S., Lopez-Montes, A., Gedil, M., Bhattacharjee, R., Girma Tessema, G., Takagi, H., Asiedu, R. and Terauchi, R., Abstract in First Global Conference on Yam 'to harness research innovations to unleash the potential of yam', 3-6 October, 2013 Accra, Ghana, 2013.
Yam (Dioscorea rotundata Poir. and D. alata L.) meristem culture - optimization and genetic stability study of micropropagated plants, Adeniyi, O. J., Adetimirin, V.*, Ingelbrecht, I. and Asiedu, R., in: 9th Triennial Symposium of the International Society for Tropical Root Crops-Africa Branch (ISTRC-AB); October 31 to November 5, 2004, Mombasa, Kenya, 2004.
Yam (Dioscorea rotundata Poir. and Dioscorea alata L.) meristem culture optimization and phenotypic stability of micropropagated plants, Adeniyi, O. J., Adetimirin, V.*, Ingelbrecht, I. and Asiedu, R., in: Proceedings of 9th ISTRC-AB symposium, 1 - 5 November 2004, pages 316-323, Mombasa, Kenya, 2007.
Yam (Dioscorea ssp.) domestication by the Nago and Fon ethnic groups in Benin, Mignouna, H. and Dansi, A.*, in: Genetic Resources and Crop Evolution, volume 50, pages 519-528, ISSN 0925-9864, 2003.
Yam (Dioscorea) husbandry: cultivating yams in the field or greenhouse, Mignouna, H. D.*, Abang, M. M., Asiedu, R. and Geeta, R., in: Cold Spring Harbor Protocols, volume 4, number 11, pages 1-3, ISSN 1559-6095, 2009. [DOI]
Yield and micronutrient uptake of white yam (Dioscorearotundata L. Poir) as influence by organomineral fertilizer ammendment on an alfisol in Ibadan South western Nigeria, Lawal, O. I., Adeoye, G., Asiedu, R., Ojeniyi, S. O., Atayese, M. O. and Olaiya, A.O.*, Abstract, P. 164 in the book of abstracts and programme of the 12th Triennial Symposium of International Society for Tropical Root Crops-Africa Branch (ISTRC-AB), 30 - 5, October, Alisa Hotel, Accra, Ghana, 2013.
YIIFSWA research brief: improving yam micropropagation series 2. Development of micropropagation system for yam (Dioscorea spp.) using somatic embryogenesis, Ossai, C., Balogun, M., Maroya, N. and Asiedu, R., YIIFSWA-II research brief: series 2, 2018.

Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement, Nyaboga, E., Tripathi, J., Manoharan, R. and Tripathi, L., in: Frontiers in Plant Science, volume 5, number 463, pages 1-14, ISSN 1664-462X, 2014. [DOI]

Keywords:

Dioscorea rotundata; Marker genes; Reporter genes; Agrobacterium tumefaciens; Genetic transformation

Development of mapping populations for genetic analysis in yams (Dioscorea rotundata and D. alata), Sartie, A. M. and Asiedu, R., Abstract (Oral presentation) in Book of Abstracts of the 11th Triennial Symposium of ISTRC-AB, held at Memling Hotel, Kinshasa. 4-8 October, 2010.

Keywords:

Dioscorea alata, Dioscorea rotundata, Hybrid identification, Molecular markers, Trait mapping, Yam

Effects of two processing methods on some nutrients and anti-nutritional factors in yellow yam (Dioscorea cayenensis), Akin-Idowu, P., Asiedu, R., Maziya-Dixon, B., Odunola, A.* and Uwaifo, A.*, in: African Journal of Food Science, volume 3, number 1, pages 022-025, ISSN 1996-0816, 2009.

Keywords:

Antinutrients; boiling; Dioscorea cayenensis; phytic acid; pounding and tannin

Secondary metabolite profile and phytotoxic activity of genetically distinct forms of Colletotrichum gloeosporioides from yam (Dioscorea spp.), Abang, M., Abraham, W., Asiedu, R., Hoffmann, P., Wolf, G. and Winter, S., in: Mychological Research, volume 113, pages 130-140, ISSN 0953-7562, 2009.

Keywords:

Anthracnose; chemotype; chromatography; colletotrichum gloeosporioides; Dioscorea spp.; mass spectrometry; metabolite profiling; NMR; phytotoxin

Sensory evaluation: the last hurdle in varietal development of yams (Dioscorea rotundata, poir) in Ghana, Otoo, E.* and Asiedu, R., in: African Journal of Biotechnology, volume 8, number 21, pages 5747-5754, ISSN 1684-5315, 2009.

Keywords:

Dioscorea; Enzymatic oxidation; farmer participatory breeding; Ghana; yams

Evaluation of water yam (Dioscorea alata L.) germplasm for reaction to yam anthracnose and virus diseases and their effect on yield, Egesi, C. N.*, Odu, B., Ogunyemi, S., Asiedu, R. and Hughes, J., in: Journal of Phytopathology, volume 155, number 9, pages 536-543, 2007.
Abstract: Use of genetic resistance is the most practical and economic way to manage major diseases of yams. In a search for sources of resistance, 40 water yam (Dioscorea alata L.) accessions from Benin, Ghana, Nigeria and Puerto Rico were screened under natural disease infection conditions in Ibadan, Nigeria. The accessions were evaluated at 1, 3 and 6 months after planting (MAP) for severity of yam anthracnose and viral diseases. The effect of the pathogens on yield was also evaluated at harvest 9 MAP. There were significant differences (P < 0.001) between accessions for severities of anthracnose and viral diseases. Eight (20%) of them had lower anthracnose area under disease progress curves (AUDPC) values than the resistant check while 10 (25%) had AUDPC values below the trial mean. There were significant variations (P < 0.001) in yield components among the accessions. There was significant negative correlation of anthracnose severity with fresh tuber yield (r = -0.51) and with number of tubers per plot (r = -0.40). Similarly, significant negative correlations were observed of virus disease severity with fresh tuber yield (r = -0.78) and number of tubers per plot (r = -0.65). Linear regression models also showed that the fresh yield had significant negative relationships with anthracnose (R2 = 0.26) and viral (R2 = 0.62) diseases. The accessions identified as resistant constitute a valuable resource for breeding of resistant germplasm.
First report of cucumber mosaic virus in yams (Dioscorea spp.) in Ghana, Togo and Republic of Benin in West Africa, Eni, A., Kumar, P. L., Asiedu, R., Alabi, O., Naidu, R., Hughes, J. and Rey, M., in: Plant Disease, volume 92, number 5, pages 833, ISSN 0191-2917, 2008. [DOI]
Abstract: Yam (Dioscorea spp., family Dioscoreaceae) is one of the most important food crops cultivated in the West African yam zone comprising the forest and savannah areas of Nigeria, Ghana, Cote d'Ivoire, Republic of Benin, and Togo, which account for more than 90% of the 4.59 million ha of yam cultivation worldwide (1). A survey was conducted in 2005 to document viruses in yams in Ghana, Togo, and the Republic of Benin. Samples (1,405) from five species of yam showing mosaic, chlorosis, and stunting as well as asymptomatic plants were tested for Dioscorea bacilliform virus (DBV, genus Badnavirus), Yam mosaic virus (YMV, genus Potyvirus), and Yam mild mosaic virus (YMMV, genus Potyvirus), the three most common viruses infecting yams. In addition, samples were tested for Cucumber mosaic virus (CMV), since CMV was previously reported to infect yams in Cote d'Ivoire (2) and Nigeria (3). In protein-A sandwich-ELISA with polyclonal antibodies to a cowpea isolate of CMV, 23 of the 1,405 samples (6 of 218 samples from Togo, 13 of 628 samples from Ghana, and 4 of 559 samples from Republic of Benin) tested positive for CMV. The CMV-positive samples were from D. alata (N = 16) and D. rotundata (N = 7), whereas all samples from D. cayenensis, D. dumetorum, and D. bulbifera tested negative. CMV was detected as mixed infections with DBV, YMV, or YMMV in 21 of 23 samples. Some of these samples showed puckering, chlorosis, mottling, and crinkling, whereas some plants infected by two or more viruses were asymptomatic. Only two samples from D. rotundata had a single infection of CMV and they showed mild chlorotic symptoms in young leaves that were inconspicuous in mature leaves. In sap inoculations, the virus induced systemic mosaic in Nicotiana glutinosa. The presence of CMV in ELISA-positive yam samples was further confirmed by immunocapture-reverse transcription (IC-RT)-PCR using CMV antibodies as trapping antibody and oligonucleotide primers specific for a 485 nt corresponding to 3' end of the coat protein gene and C-terminal noncoding region of RNA-3 (4). To confirm the specificity of ICRT-PCR, the 485-bp amplicons from an isolate from the Republic of Benin was cloned into pCR2.1 (Invitrogen, Carlsbad, CA) and three independent clones were sequenced from both orientations. Pairwise comparison of a consensus sequence (Accession No. EU274471) with corresponding sequences of other CMV isolates deposited in GenBank showed 99% identity at the nucleotide sequence level (Accession No. U22821) and revealed that the CMV isolate from yam belongs to sub-Group IA. To our knowledge, this is the first report of CMV infection in yams (D. alata and D. rotundata) in Ghana, Togo, and the Republic of Benin. Together with a previous documentation of CMV in D. alata and D. trifida in Cote d'Ivoire and Nigeria (2,3), this report adds to existing knowledge on distribution of CMV in yams with implications for yam production and germplasm distribution in the West Africa Region.
Severity of anthracnose and virus diseases of water yam (dioscorea alata L.) in NIgeria I: effects of yam genotype and date of planting, Egesi, C. N.*, Onyeka, T. and Asiedu, R., in: Crop Protection, volume 26, number 8, pages 1259-1265, ISSN 0261-2194, 2007.
Abstract: Anthracnose and virus diseases exert devastating impacts on yam production in many tropical regions of the world where the crop contributes to food security and income generation. The complexities in their epidemiology necessitate the use of integrated approaches in their management. Six genotypes of Dioscorea alata, planted on six dates (March?August) in each of 1998 and 1999 in Ibadan, Nigeria, were evaluated to determine the effects of planting date and genotype on severity of the two diseases. The date of planting had a profound influence on anthracnose severity, contributing 72% of the observed variation due to combined effects of planting date, genotype and their interactions. In contrast, the genotype effect was most dominant with respect to virus severity, accounting for 85% of the total observed variation. Planting in August supported least anthracnose development while April and May plantings resulted in the least severity of virus diseases. While selection of planting time could be used to manage anthracnose disease, its application could be influenced by the prevailing weather conditions in a particular location. Two of the genotypes (TDa 291 and TDa 297) had low severity values for both diseases across the different planting dates. Availability of such sources of multiple disease resistance and appropriate choice of planting date would be very useful in integrated management of the two major yam diseases.

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