Titles (18)Keywords (1)Abstracts (2)
A high-throughput regeneration and transformation platform for production of genetically modified banana, Tripathi, J., Oduor, R. O. and Tripathi, L., in: Frontiers in Plant Science, volume 6, number 1025, pages 1-13, ISSN 1664-462X, 2015. [DOI]
 
A simple and rapid protocol for the genetic transformation of Ensete ventricosum, Matheka, J. M., Tripathi, J., Merga, I. F., Gebre, E.* and Tripathi, L., in: Plant Methods, volume 15, number : 130, pages 1-17, ISSN 1746-4811, 2019. [DOI]
 
Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement, Nyaboga, E., Tripathi, J., Manoharan, R. and Tripathi, L., in: Frontiers in Plant Science, volume 5, number 463, pages 1-14, ISSN 1664-462X, 2014. [DOI]
 
Effects of various virulent strains of Agrobacterium tumefaciens on genetic transformation of banana (Musa sp.) cultivar williams, Tripathi, L., Esuola, C. and Fawole, I.*, in: African Journal of Horticultural Science, volume 5, pages 84-91, ISSN 1998-9326, 2011.
 
Genetic transformation in Musa species (Banana), Sagi, L., Remy, S., Verelst, B., Swennen, R.+ and Panis, B., in: Biotechnology in Agriculture and Forestry, volume 34, pages 214-227, 1995.
 
Genetic transformation of African farmer-preferred cassava (Manihot esculenta Crantz) cultivars, Nyaboga, E., Njiru, J., Vanderschuren, H., Nguu, E.* and Tripathi, L., Abstract, S06-03 in Program and Abstracts, Second Scientific Conference of the Global Cassava Partnership for the 21st Century (GCP21-II). June 18-22, Kampala, Uganda., 2012.
 
Genetic transformation of African farmer-preferred cassava cultivars, Nyaboga, E., Njiru, J., Vanderschuren, H., Taylor, N., Nguu, E.*, Gruissem, W. and Tripathi, L., Abstract, P. 66 in Abstract of papers of the International Confernece on Tropical Roots and Tubers for Sustainable Livelihood under changing agro-climate (ICTRT) 9-12 July 2013, Hotel Mascot, Thiruvananthapuram, Kerala, 2013.
 
Genetic transformation of banana and plantain (Musa spp.) via particle bombardment, Sagi, L., Panis, B., Remy, S., Schoofs, H., De Smet, K., Swennen, R.+ and Cammue, B. P. A., in: Nature Biotechnology, volume 13, pages 481-485, 1995.
 
Genetic transformation of banana for disease resistance, Tripathi, L., Tripathi, J. and Tushemereirwe, W. K.*, Abstract in Book of Abstracts of the International Conference 'Plant Transformation Technologies II', Vienna, February 19th - 22nd, 2011.
 
Genetic transformation of bananas for resistance to Xanthomonas wilt disease, Tripathi, L., Abstract, P. 50 in the programme and abstracts book of the 2nd National Biosafety Conference, 5-9 August, 2013, Kenyatta International Convention Centre (KICC), Nairobi, Kenya, 2013.
 
Genetic transformation of cassava - independent of genotype, Jorgensen, K., Ingelbrecht, I., Jensen, S., Olsen, E., Sorensen, C., Kannangara, R. and Moller, B., 2008.
 
Genetic transformation of cowpea (Vigna unguiculata L. Walp) via Agrobacterium tumefaciens using embryonic axis explants: proceedings, Raji, A., Odeseye, B., Oriero, C., Akinyemi, J., Odunlami, T., Odeyemi, F. and Ingelbrecht, I., in: Joint Annual Meeting of the American Society of Plant Biologists, Canadian Society of Plant Physiologists, Sociiete Canadienne de Physiologie Vegetale, August 5-9 2006, Boston, Massachusetts, USA, 2006.
 
Genetic transformation of geraniums, Pellegrineschi, A., Biotechnology in agriculture and forestry, No. 38, pages 211-221, Springer-Verlag, 1996.
 
Genetic transformation of Musa, Tripathi, L., in: Paper presented at the Workshop on Strategies for Banana Transformation to Address the Major Constraints to Banana and Plantain Production in Africa, August, 2003.
 
Plant regeneration and genetic transformation, Kuta, D., Mbanasor, E.*, Raji, A. and Ingelbrecht, I., 2005.
 
Regeneration and genetic transformation in cowpea, Machuka, J., Adesoye, A. and Obembe, O.*, 2002.
 
Somatic embryogenesis and genetic transformation of African farmer-grown cassava genotypes that are susceptible to the Cassava Brown Streak Disease, Ingelbrecht, I., Raji, A., Oyelakin, O., Winter, S., Moller, B., Dixon, A. and Jorgensen, K., 2008.
 
Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmer-preferred cassava cultivars, Nyaboga, E., Njiru, J., Nguu, E.*, Gruissem, W., Vanderschuren, H. and Tripathi, L., in: Frontiers in Plant Science, volume 4, number 526, pages 1-11, ISSN 1664-462X, 2013. [DOI]
 
Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement, Nyaboga, E., Tripathi, J., Manoharan, R. and Tripathi, L., in: Frontiers in Plant Science, volume 5, number 463, pages 1-14, ISSN 1664-462X, 2014. [DOI]
 
Keywords:Dioscorea rotundata; Marker genes; Reporter genes; Agrobacterium tumefaciens; Genetic transformation

Genetic transformation of cassava - independent of genotype, Jorgensen, K., Ingelbrecht, I., Jensen, S., Olsen, E., Sorensen, C., Kannangara, R. and Moller, B., 2008.
 
Abstract:
Cassava is a vegetatively propagated crop and its improvement through conventional breeding is challenging due to its high heterozygosity and low fertility. As it has not been possible to solve all cassava's problems connected to agriculture and consumption by traditional breeding, another solution could be to use molecular breeding. Major deficits of cassava are low protein content in the tubers, rapid post-harvest tuber deterioration and high content of cyanogenic glucosides. Careful processing of cassava roots is required to remove the released hydrogen cyanide which can cause acute or chronic cyanide intoxication. Unfortunately, processing to remove hydrogen cyanide typically results in loss of protein, minerals, and vitamins. For successfull molecular breeding of cassava, a genotype-independent genetic transformation method is essential. So far it has only been possible to transform model lines which have limited agricultural importance. Here a regeneration and transformation method is presented which has been successfully applied to all African varieties tested so far with a transformation frequency ranging from 0.2% to 3.8%. The method is based on the procedure developed by Li et al. (1996). This method is among others now used to 1) produce acyanogenic cassava (J{\o}rgensen et al 2005), 2) improve the nutritional value in the tubers, 3) virus resistance. The improvement of the nutritional value is focused on increasing the protein content in the tubers in varieties with and without a naturally increased levels of pro-vitamin A.

Somatic embryogenesis and genetic transformation of African farmer-grown cassava genotypes that are susceptible to the Cassava Brown Streak Disease, Ingelbrecht, I., Raji, A., Oyelakin, O., Winter, S., Moller, B., Dixon, A. and Jorgensen, K., 2008.
 
Abstract:
Cassava is one of the most important sources of carbohydrates for over 500 million people in the (sub)tropics. Cassava plays an important role in the food security of many developing nations, especially in sub-Saharan Africa (SSA). Cassava is highly heterozygous, genetically complex, and many varieties either do not flower or produce few seeds thus hampering conventional crop improvement. Several major constraints limit the production and utilization of cassava roots, including two viral diseases, the Cassava Brown Streak Disease (CBSD) and the Cassava Mosaic Disease (CMD), which are specific to SSA. Farmer-preferred landraces are often susceptible to CBSD and/or CMD. Genetic transformation of cassava could complement conventional breeding programs for CBSD and/or CMD resistance. Current protocols for genetic transformation of cassava are limited to model genotypes which are not used by farmers of breeders in SSA. Since transformation protocols are genotype-dependent, suitable procedures for genetic modification of the landraces need to be developed. We have established somatic embryogenesis and organogenesis for three farmer/breeder-preferred varieties, two from East Africa (cv Kibaha and cv Albert) and one from West Africa (TME12) which are susceptible to CBSD. Primary and cyclical somatic embryogenesis was established for the three varieties. Cotyledon tissues from somatic embryos were used as source explants for Agrobacterium-mediated genetic transformation. Using an intron-interrupted {\^a}{\"i}¿½glucuronidase reporter gene construct under control of the Cassava vein mosaic virus promoter, stably transformed cassava tissues and plants were obtained. Molecular evidence for stable expression of the transgenes will be presented.