Titles (85)Keywords (1)Abstracts (5)
Additive yield response of chickpea (Cicer arietinum L.) to rhizobium inoculation and phosphorus fertilizer across smallholder farms in Ethiopia, Wolde-Meskel, E., van Heerwaarden, J., Abdulkadir, B., Kassa, S.*, Aliyi, I.*, Degefu, T., Wakweya, K.*, Kanampiu, F. and Giller, K., in: Agriculture, Ecosystems and Environment, volume 261, pages 144-152, ISSN 0167-8809, 2018. [DOI]
 
Arbuscular mycorrhizal fungi (AMF) respond to rhizobial inoculation and cropping systems in farmer's fields in the Guinea savanna, Sanginga, N., Carsky, R. and Dashiell, K., in: Biology and Fertility of Soils, volume 30, number 3, pages 179-186, ISSN 0178-2762, 1999.
 
Assessment of inoculation methods in evaluating response of yam cultivars to infection by Scutellonema bradys, Baimey, H., Coyne, D. and Labuschagne, N.*, in: Nematology, volume 7, number 3, pages 375-379, ISSN 1388-5545, 2005.
 
Benefits of inoculation, P fertilizer and manure on yields of common bean and soybean also increase yield of subsequent maize, Rurangwa, E.*, Vanlauwe, B. and Giller, K., in: Agriculture, Ecosystems and Environment, pages 1-13, ISSN 0167-8809, 2017. [DOI]
 
Biolistic inoculation of cassava mosaic begomoviruses to screen for resistance in cassava: Book of program, Ariyo, O., Koerbler, M., Atiri, G.* and Winter, S., in: Paper presented at: 13th Symposium of the International Society of Tropical Root Crops (ISTRC); 10-14 November, International Conference Center, Arusha, Tanzania, 2003.
 
Breeding implications of nodulation performance and root structure under natural inoculation for soil fertility enhancement and sustainable cowpea production, Mekonnen, T. W.*, Mekbib, F.*, Amsalu, B.*, Gedil, M. and Labuschagne, M.*, in: Frontiers in Sustainable Food Systems, volume 6, number -: 1076760, pages 1-14, ISSN 2571-581X, 2022. [DOI]
 
Chlorophyll fluorescence analysis for assessing water deficit and Arbuscular Mycorrhizal Fungi (AMF) inoculation in cassava (Manihot esculenta Crantz), Oyetunji, O.*, Ekanayake, I. J. and Osonubi, O.*, in: Advances in Biological Research, volume 1, number 3-4, pages 108-117, ISSN 1992-0067, 2007.
 
Co-inoculation effect of rhizobia and plant growth promoting rhizobacteria on common bean growth in a low phosphorus soil, Korir, H.*, Mungai, N. W.*, Thuita, M., Hamba, Y.* and Masso, C., in: Frontiers in Plant Science, volume 8, number 141, pages 1-10, ISSN 1664-462X, 2017. [DOI]
 
Comparison of field inoculation methods for screening maize against downy mildew (Perononsclerospora sorghi), Cardwell, K., Kling, J. and Bock, C., in: Plant Breeding, volume 116, pages 221-226, 1997.
 
Comparison of varietal reactions to cowpea anthracnose in field plot and detached petiole in inoculations, Williams, R. J., Abstracts of papers presented at 66th Annual Meeting of A.P.S. and 40th Session Canadian Phytopathological Society, Vancouver, Canada. p.87, 1974.
 
Cowpea nodulation and response to inoculation in West Africa, Mulongoy, K., Ayanaba, A., Asanuma, S. and Ranga Rao, V., in: Paper presented at 1st OAU/STRC Inter-African Conference on Bio-fertilizers,22-26 March 1982, 1982.
 
Defense-related gene expression in susceptible and tolerant bananas (Musa spp.) following inoculation with non-pathogenic Fusarium oxysporum endophytes and challenge with Radopholus similis, Paparu, P., Dubois, T., Coyne, D. and Viljoen, A.*, in: Physiological and Molecular Plant Pathology, volume 71, number 4-6, pages 149-157, ISSN 0885-5765, 2007.
 
Dependency of promiscuous soybean and herbaceous legumes on arbuscular mycorrhizal fungi and their response to bradyrhizobial inoculation in low P soils, Nwoko, H. and Sanginga, N., in: Applied Soil Ecology, volume 13, number 3, pages 251-258, 1999. [DOI]
 
Dual inoculation of Fusarium oxysporum endophytes in banana: effect on plant colonization, growth and control of the root burrowing nematode and the banana weevil, Paparu, P., Dubois, T., Coyne, D. and Viljoen, A.*, in: Biocontrol Science and Technology, volume 19, number 6, pages 639-655, ISSN 1360-0478, 2009.
 
Effect of dual endophyte inoculations on plant colonization, and control of Radopholus similis and the banana weevil, Paparu, P., Dubois, T., Coyne, D. and Viljoen, A.*, Abstracts, P. 103 in the Program and Book of Abstracts, International Banana 2008 Conference, "Banana and Plantain in Africa: Harnessing International Partnerships to Increase Research Impact". 5 - 9 October, Mombasa, Kenya, 2008.
 
Effect of fertilizatin and Rhizobium inoculation on the growth of Leucaena and Gliricidia on an Alfisol in south-western Nigeria, Cobbina, J., Mulongoy, K. and Atta-Krah, A. N., pages 161-169, John Wiley & Sons; Sayce Publishing; international Institute of Tropical Agriculture (IITA); AABNF, 1992.
 
Effect of Fusarium oxysporum endophyte inoculation on the activities of phenylpropanoid pathway enzymes and Radopholus similis numbers in susceptible and tolerant East African Highland bananas, Paparu, P., Dubois, T., Coyne, D. and Viljoen, A.*, in: Nematology, volume 12, number 3, pages 469-480, ISSN 1388-5545, 2010.
 
Effect of inoculation and mineral nutrients on nodulation and growth of Leucaena leucocephala (Lam.) de Wit, Mulongoy, K., Sanginga, N. and Ayanaba, A., 1984.
 
Effect of inoculation and mineral nutrients on nodulation and growth of Leucaena leucocephala (Lam.) de Wit, Mulongoy, K., Sanginga, N. and Ayanaba, A., 1984.
 
Effect of inoculation and nitrogen fertilizer on soybean in western Nigeria, Kang, B. T., in: Experimental Agriculture, volume 11, number 1, pages 23-31, 1975.
 
Effect of inoculation with Rhizobium, P application and liming on early growth of leucaena (Leucaena leucocephala Lam. de Wit), Gichuru, M. and Mulongoy, K., volume 2, pages 72-80, Collection Actes de l'ISRA, 1990.
 
Effect of lime and Rhizobium inoculation on the yield of cowpea in heavy rainfall, acid soil location, Rao, R. V. and Pulver, E. L., 1980.
 
Effect of phosphate fertilization and inoculation with VA-mycorrhizal fungi on performance of cassava (Manihot esculenta Crantz) grown on an Alfisol, Kang, B. T., Islam, R., Sanders, F. E. and Ayanaba, A., in: Field Crops Research, volume 3, pages 83-94, 1980.
 
Effect of Rhizobium inoculation on field- grown soybeans in Western Nigeria and assessment of inoculum persistence during a two-year fallow, Ranga Rao, V., Ayanaba, A., Eaglesham, A.R.J. and Thottappilly, G., in: Tropical Agricultural, volume 62, number 2, pages 125-130, 1985.
 
Effect of single or dual inoculation of the arbuscular mycorrhizal fungus Glomus mosseae and root-nodulating rhizobacteria on reproduction of the burrowing nematode Radopholus similis on non-leguminous and leguminous banana intercrops, Van der Veken, L., Cabasan, M. T. N.*, Elsen, A., Swennen, R. and De Waele, D ., in: Journal of Plant Diseases and Protection, pages 1-11, ISSN 1861-3829, 2021. [DOI]
 
Effect of temperature, humidity and inoculation method on susceptibility of Schistocerca gregaria to Metarhizium flavoride, Fargues, J., Ouedraogo, A., Goettel, M. S. and Lomer, C., in: Biocontrol Science and Technology, volume 7, pages 345-356, 1997.
 
Effect of two species of arbuscular mycorrhizal fungi inoculation on development of micro-propagated yam plantlets and suppression of Scutellonema bradys (Tylenchideae), Tchabi, A.*, Hountondji, F.*, Ogunsola, B., Lawouin, L., Coyne, D., Wiemken, A. and Oehl, F., in: Journal of Entomology and Nematology, volume 8, number 1, pages 1-10, ISSN 2006-9855, 2016. [DOI]
 
Effects of arbuscular mycorrhizal inoculation and phosphorus application on yield and nutrient uptake of yam, Dare, M. O.*, Abaidoo, R., Fagbola, O.* and Asiedu, R., in: Communications in Soil Science and Plant Analysis, volume 41, pages 2729-2743, ISSN 0010-3624, 2010.
 
Effects of inoculation and nitrogen fertilizer on soybeans in Western Nigeria, Kang, B. T., in: Experimental Agriculture, volume 11, number 1, pages 23-31, ISSN 0014-4797, 1975. [DOI]
 
Evaluating host plant reaction of Musa germplasm to Radopholus similis by inoculation of single primary roots, De Schutter, B., Speijer, P., Dochez, C., Tenkouano, A. and De Waele, D., in: Nematropica, volume 31, number 2, pages 295-300, 2001.
 
Evaluation of an inoculation method for screening cassava against genotypes against cassava mosaic disease, Ariyo, O., Koerbler, M., Atiri, G.*, Winter, S. and Dixon, A., in: Paper presented at: American Phytopathological Society Meeting, 2003.
 
Feasibility of transference of inoculation-related technologies: a case study of evaluation of soybean rhizobial strains under the agro-climatic conditions of Brazil and Mozambique, Chibeba, A. M., Boahen, S., de Fatima Guimaraes, M.*, Nogueira, M. A.* and Hungria, M.*, in: Agriculture, Ecosystems and Environment, pages 1-11, ISSN 0167-8809, 2017. [DOI]
 
Field based assessment of Capsicum annuum performance with inoculation of rhizobacterial consortia, Kaushal, M., Mandyal, P.* and Kaushal, R.*, in: Microorganisms, volume 7, number -: 89, pages 1-12, ISSN 2076-2607, 2019. [DOI]
 
Groundnut genotypes response to Rhizobium inoculation in the Sudan Savanna of Nigeria, Umar, F. G.*, Abdelgadir, A. H., Yusuf, A. A.*, Jibrin, J. M.*, Motagi, B. N. and Kamai, N., Abstract, p. 147, in the book of abstracts of the PanAfrican Grain Legume and World Cowpea Conference 28 February - 4 March, Livingstone, Zambia, 2016.
 
Growth and yield responses of cowpea to inoculation and phosphorus fertilization in different environments, Boahen, S., Engoke, C., Chikoye, D. and Abaidoo, R., in: Frontiers in Plant Science, volume 8, number 646, pages 1-13, ISSN 1664-462X, 2017. [DOI]
 
Growth and yield responses of two soybean cultivars to Inoculation, P and N fertilization in Northern Mozambique, Boahen, S., Chikoye, D., Abaidoo, R., Muananamuale, C. and Engoke, C., 2014.
 
Growth and yield responses of two soybean cultivars to Inoculation, P and N fertilization in Northern Mozambique, Boahen, S., Chikoye, D., Abaidoo, R., Muananamuale, C. and Engoke, C., Abstract (Poster), presentation at the ASA, CSSA, & SSSA International Annual Meeting held Nov. 2-5, at Long Beach, 2014.
 
Growth of Gliricidia and Leucaena as affected by Rhizobium inoculation and N and P fertilizar application in Nigeria, Onafeko, O., Kang, B. and Omueti, J. A.*, in: Alley farming research and development: proceedings of an international conference on alley farming, 14-18 September 1992, pages 226-235, 1995.
 
Host response to black sigatoka in Musa germplasm of different ages under natural inoculation conditions, Mobambo, K. N., Pasberg-Gauhl, C., Gauhl, F. and Zuofa, K. E., in: Crop Protection, volume 16, pages 359-363, 1997.
 
Identification of levels of resistance to cassava root rot disease (Botryodiplodia theobromae) in African landraces and improved germplasm using In vitro inoculation method, Onyeka, T., Dixon, A. and Ekpo, E.*, in: Euphytica, volume 145, number 3, pages 281-288, ISSN 0014-2336, 2005.
 
Impact of timing and method of virus inoculation on the severity of wheat streak mosaic disease, Wosula, E. N., McMechan, A. J., Knoell, E., Tatineni, S., Wegulo, S. N. and Hein, G. L., in: Plant Disease, volume 102, number 3, pages 645-650, ISSN 0191-2917, 2018. [DOI]
 
Increasing cowpea productivity combining rock phosphate and arbuscular mycorrhizal fungi inoculation in sub-Saharan Africa: proceeding/poster, Suzuki, K., Fatokun, C. and Boukar, O., Tropentag - University of Bonn, 2017.
 
Influence of P sources and rhizobium inoculation on growth and yield of soybean genotypes on Ferric Lixisols of northern Guinea savanna zone of Ghana, Adjei-Nsiah, S., Kumah, J. F.*, Owusu-Bennoah, E.* and Kanampiu, F., in: Communications in Soil Science and Plant Analysis, pages 1-16, ISSN 0010-3624, 2019. [DOI]
 
Inoculation of legumes - Zimbabwe, Chabata, I. and N2Africa, , 2014.
 
Inoculation of Leucaena leucocephala (Lam.) de Wit. with Rhizobium and its nitrogen contribution to a subsequent maize crop, Mulongoy, K., Sanginga, N. and Ayanaba, A., Ashford, 1984.
 
Inoculation of Leucocephala(Lam.) de Wit with Rhizobium and its nitrogen contribution to a subsequent maize crops, Sanginga, N., Mulongoy, K. and Ayanaba, A., in: Biological Agriculture and Horticulture, volume 3, pages 347-352, ISSN 0144-8765, 1986.
 
Inoculation studies on three ear rot diseases of tropic maize, Fajemisin, J., Durojaiye, J. A. T., Kim, S. K. and Efron, Y., in: Abstracts of the American Phytopathological Society, number 479, 1987.
 
Inoculation with indigenous rhizobial isolates enhanced nodulation, growth, yield and protein content of soybean (Glycine max L.) at different agro-climatic regions in Ethiopia, Abera, Y.*, Masso, C. and Assefa, F.*, in: Journal of Plant Nutrition, volume 42, number 16, pages 1900-1912, ISSN 0190-4167, 2019. [DOI]
 
Inoculation, colonization, and distribution of fungal endophytes in Musa tissue culture plants, Paparu, P., Dubois, T., Gold, C., Adipala, E.*, Niere, B. and Coyne, D., in: Ugandan Journal of Agricultural Sciences, volume 9, number 1, pages 583-589, 2004.
 
Is there a need for Bradyrhizobium yuanmingense and B. japonicum reinoculation in subsequent cropping seasons under smallholder farmers' conditions?, Ulzen, J.*, Abaidoo, R. C.*, Masso, C., Owusu-Ansah, E. D.* and Ewusi-Mensah, N.*, in: Applied Soil Ecology, volume 128, pages 54-60, ISSN 0929-1393, 2018. [DOI]
 
Legume inoculation technology manual: putting nitrogen fixation to work for smallholder farmers in Africa, Abaidoo, R. C.*, Ewusi-Mensah, N.*, Opoku, A.*, N2Africa, and IITA, , N2Africa, 2013.
 
Legume inoculation technology manual: putting nitrogen fixation to work for smallholder farmers in Africa, IITA, , 2014.
 
L'inoculation des legumineuses en Afrique, Mulongoy, K., 1984.
 
Nodulation and growth of Leucaena leucocephala (Lam.) de Wit as affected by inoculation and N fertilizer, Sanginga, N., Mulongoy, K. and Ayanaba, A., in: Plant and Soil, volume 112, pages 129-135, 1988.
 
Nodulation of soybean cultivars with Rhizobium spp. and their response to inoculation with R. japonicum, Pulver, E. L., Brockman, F. and Wien, H. C., in: Crop Science, volume 22, pages 1065-1070, ISSN 0011-183X, 1982.
 
On-farm evaluation and determination of sources of variability of soybean response to Bradyrhizobium inoculation and phosphorus fertilizer in northern Ghana, Ulzen, J.*, Abaidoo, R., Ewusi-Mensah, N.* and Masso, C., in: Agriculture, Ecosystems and Environment, volume 267, pages 23-32, ISSN 0167-8809, 2018. [DOI]
 
Persistence and recovery of introduced Rhizobium ten years after inoculation on Leucaena leucocephala grown on an Alfisol in southwetern Nigeria, Sanginga, N., Danso, S. K. A.*, Mulongoy, K. and Ojeifo, A. A., in: Plant and Soil, volume 159, pages 199-204, 1994.
 
Photosynthetic photochemical response of cassava Manihot esculenta Crantz to water stress and mycorrhizal inoculation [abstract], Oyetunji, O.*, Ekanayake, I. J. and Osonubi, O.*, in: Tropical Agriculture (Trinidad), volume 75, pages 328-329, 1998.
 
Physiological evaluation of soybean (Glycine max (L.) Merr.) varieties as influenced by inoculation and sowing date in the Guinea Savanna, Aminu, A. S.*, Ahmadu Bello University, 2019.
 
Physiological responses of cowpea (Vigna unguiculata L. Walp) varieties to Rhizobia inoculation, phosphorus application and sequential cropping system in Minna, Nigeria, Adediran, O. A.*, Federal University of Technology, 2019.
 
Relative usefulness of mechancial and aphid inoculation as modes of screening cowpeas for resistance against cowpea aphid-borne mosaic virus, Atiri, G.* and Thottappilly, G., in: Tropical Agriculture (Trinidad), volume 61, number 4, pages 289-292, 1984.
 
Response of common bean (Phaseolus vulgaris L.) to nitrogen, phosphorus and rhizobia inoculation across variable soils in Zimbabwe, Chekanai, V.*, Chikowo, R.* and Vanlauwe, B., in: Agriculture, Ecosystems and Environment, volume 266, pages 167-173, ISSN 0167-8809, 2018. [DOI]
 
Response of cowpea (Vigna unguiculata) to inoculation with VA-mycorrhizal fungi and to rock phosphate fertilization in some unsterilized Nigerian soils, Islam, R., Ayanaba, A. and Sanders, F. E., in: Plant and Soil, volume 54, pages 107-117, 1980.
 
Response of cowpea (Vigna unguiculata) to VA-mycorhizal inoculation and rock phosphate fertilization in five unsterile Nigerian soils, Islam, R., Ayanaba, A. and Sanders, F. E., 1976.
 
Response of grain legumes to rhizobial inoculation in two savanna soils of Nigeria, Aliyu, I.*, Yusuf, A. A.* and Abaidoo, R., in: African Journal of Microbiology Research, volume 7, number 15, pages 1332-1342, ISSN 1996-0808, 2013. [DOI]
 
Response of Mucuna pruriens to symbiotic nitrogen fixation by rhizobia following inoculation in farmers' fields in the derived savanna of Benin, Houngnandan, P.*, Sanginga, N., Vanlauwe, B., Woomer, P.* and Van Cleemput, O., in: Biology and Fertility of Soils, volume 30, pages 558-565, ISSN 0178-2762, 2000. [DOI]
 
Response of promiscuous soybean to rhizobial inoculation in combination with organic and mineral fertilizers in some soils of the Nigeria Guinea Savanna, Ekaette, J. E., Federal University of Technology, 2017.
 
Response of promiscuous-nodulating soybean (Glycine max L. Merr.) genotypes to Bradyrhizobium inoculation at three field sites in Mozambique, Gyogluu, C.*, Boahen, S. and Dakora, F. D.*, in: Symbiosis, pages 1-8, ISSN 0334-5114, 2016. [DOI]
 
Response of some tropical nitrogen-fixing woody legumes to drought and inoculation with mycorrhiza, Awotoye, O. O.*, Atayese, M. O.*, Osonubi, O.*, Mulongoy, K. and Okali, D. U. U., pages 67-77, John Wiley & Sons; Sayce Publishing; International Institute of Tropical Agriculture (IITA); AABNF, 1992.
 
Response of soybean to seed inoculation with rhizobium in humid tropical conditions, Ranga Rao, V., Ayanaba, A. and Eaglesham, A.R.J., 1979.
 
Response of soybean(Glycine max (L.) Merill) genotypes to inoculation with cowpea mild mottle virus (CPMMV), genus Carlavirus, Time, I., Atiri, G.* and Kumar, P. L., Abstract in Book of abstracts of the 36th annual conference of the Nigerian Society of Plant Protection (NSPP), 4 - 8 September, Futa, Nigeria., 2011.
 
Response of two Acacia species to drought and inoculation with an ectomycorrhizal fungus, Osonubi, O.* and Mulongoy, K., pages 375, Kluwer Academic Publishers, 1991.
 
Responses of white yam (Dioscorea rotundata) cultivars to inoculation with three viruses, Odu, B., Hughes, J., Asiedu, R., Ng, N. Q., Shoyinka, S.* and Oladiran, O., in: Plant Pathology, volume 53, number 2, pages 141-147, ISSN 0032-0862, 2004.
 
Seed inoculation: master farmer training practical, N2Africa, , N2Africa, 2014.
 
Soyabean response to rhizobium inoculation across sub-Saharan Africa: patterns of variation and the role of promiscuity, van Heerwaarden, J., Baijukya, F., Boahen, S., Adjei-Nsiah, S., Ebanyat, P., Kamai, N., Wolde-Meskel, E., Kanampiu, F., Vanlauwe, B. and Giller, K., in: Agriculture, Ecosystems and Environment, volume 261, pages 211-218, ISSN 0167-8809, 2018. [DOI]
 
Soybean (Glycine max L. Merrill) responds to phosphorus application and rhizobium inoculation on Acrisols of the semi-deciduous forest agro-ecological zone of Ghana, Adjei-Nsiah, S., Martei, D.*, Yakubu, S. A.* and Ulzen, J.*, in: PeerJ, pages 1-16, ISSN 2167-8359, 2022. [DOI]
 
Soybean (Glycine max) response to rhizobia inoculation as influenced by soil nitrogen levels, Nyaguthii, M. C.*, A thesis submitted in partial fulfilment of the requirements for award of degree of Masters of Science in Agronomy in the School of Agriculture and Enterprise Development, Kenyatta University, Suba North, Kenya, 2017.
 
Soybean inoculation in northern Mozambique, Muananamuale, C., Ussene, R., Boahen, S., Chikoye, D. and Dakora, F. D.*, Abstract (Poster), p. 287 in the Proceedings of the 10th African Crop Science Society Conference,"from soil to soul- crop production for improved African livelihoods and better environment for future generations", held in Maputo, Mozambique. 10-13 October, 2011.
 
Soybean yield determinants and response to rhizobial inoculation in an on-farm trial in the northern Guinea savanna of Nigeria, Okogun, J., Otuyemi, B. and Sanginga, P., in: West African Journal of Applied Ecology, volume 6, pages 30-39, 2004.
 
The effect of vesicular arbuscular mycorrhizal inoculation on nutrient uptake and yield of alley cropped cassava in a degraded Alfisol of southwestern Nigeria, Osonubi, O.*, Atayese, M. O.* and Mulongoy, K., in: Biology and Fertility of Soils, volume 20, number 1, pages 70-76, 1995.
 
The influence of arbuscular mycorrhizal fungi inoculation on micro-propagated hybrid yam (Dioscorea spp.) growth and root knot nematode (Meloidogyne spp.) suppression, Tchabi, A.*, Hountondji, F.*, Ogunsola, B., Lawouin, L., Coyne, D., Wiemken, A. and Oehl, F., in: International Journal of Current Microbiology and Applied Sciences, volume 5, number 10, pages 267-281, ISSN 2319-7692, 2016. [DOI]
 
The phosphate inhibition paradigm: host and fungal genotypes determine arbuscular mycorrhizal fungal colonization and responsiveness to inoculation in cassava with increasing phosphorus supply, Venegas, R. A. P., Lee, S. J., Thuita, M., Mlay, P. D.*, Masso, C., Vanlauwe, B., Rodriguez, A.* and Sanders, I. R., in: Frontiers in Plant Science, volume 12, number : 693037, pages 1-11, ISSN 1664-462X, 2021. [DOI]
 
The use of biolistic inoculation of Cassava Mosaic begomoviruses in screening cassava for resistance to Cassava Mosaic Disease, Ariyo, O., Atiri, G.* and Winter, S., in: Journal of Virological Methods, volume 137, number 1, pages 43-50, 2006.
 
Towards sustainable yield improvement: field inoculation of soybean with Bradyrhizobium and co-inoculation with Azospirillum in Mozambique, Chibeba, A. M., Boahen, S., de Fatima Guimaraes, M.*, Nogueira, M. A.* and Hungria, M.*, in: Archives of Microbiology, volume 2020, pages 2579-2590, ISSN 0302-8933, 2020. [DOI]
 
Use of three inoculation methods in screening cowpea genotypes for resistance to two Colletotrichum species, Adebitan, S. A., Ikotun, T.*, Dashiell, K. and Singh, S., in: Plant Disease, volume 76, number 10, pages 1025-1028, 1992.
 
Comparative importance of infection routes for banana Xanthomonas wilt and implications on disease epidemiology and management, Nakato, V., Ocimati, W., Blomme, G., Fiaboe, K. K.+ and Beed, F., in: Canadian Journal of Plant Pathology, pages 1-11, ISSN 0706-0661, 2014. [DOI]
 
Keywords:Xanthomonas campestris musacearum; banana Xanthomonas wilt; vectors; inoculation

Breeding soybeans for the tropics capable of nodulating effectively with indigenous Rhizobium spp., Kueneman, E., Root, W. R., Dashiell, K. and Hohenberg, J., in: Plant and Soil, volume 82, number 3, pages 387-396, 1984. [DOI]
 
Abstract:
Most soybean varieties fail to nodulate effectively in tropical soils unless inoculated with a competitive strain of Rhizobium japonicum. Developing countries in the tropics, with few exceptions, lack inoculant industries to produce and distribute viable inoculants to small farmers and extension programs to teach them to use inoculant. Several soybean genotypes have been identified that nodulate effectively with many strains of the cowpea inoculation group which is ubiquitous in tropical soils of Africa. Soybean genotypes that nodulate and grow well without inoculant application are called promiscuous. Methodologies for incorporation of the promiscuity character into high-yielding backgrounds are discussed.

Characterisation of resistance in cassava against viruses causing cassava mosaic and cassava brown streak disease in Africa, Koerbler, M., Stein, B., Ingelbrecht, I., Dixon, A. and Winter, S., 2008.
 
Abstract:
Cassava mosaic disease caused by diverse geminiviruses (CMG) and Cassava brown streak disease caused by cassava brown streak virus (CBSV), are key constraints to the cultivation of cassava in Sub-Sahara Africa. We have collected and characterised the diversity of the most prominent geminiviruses from all African cassava cultivation areas and Cassava brown streak viruses from East Africa, to use defined viruses for resistance studies in selected IITA cassava breeding lines and African land races. Virus infections were established in cassava by either graft inoculation with scions of virus infected plants or, by particle bombardment of cloned infectious viruses. Resistance against cassava mosaic geminiviruses was identified in several breeding lines e.g. TME 4, TMS 96/0529 and TMS 96/0160 responding with abortion of virus infections after virus introduction. Other genotypes, e.g. TMS 96/0304, became infected but recovered from symptoms but maintaining the infection status. When cassava clones were analysed for CBSV resistance, it became evident that geminivirus resistance was tightly correlated with susceptibility against CBSV. Geminivirus resistant cassava responded with often severe CBSV infections (e.g. TMS 96/0160) upon virus inoculation. Interestingly, cassava genotypes with an intermediate resistance, TMS 96/0304, initially became mixed infected with CBSV/CMG however subsequently aborting CMG, to establish single CBSV infections. Differential responses were also recorded for strains of CBSV. While CMG resistance in cassava was against all CMG, differential responses were observed in cassava inoculated with CBSV isolates obtained from Kenya and Mozambique. While the CMG susceptible cassava landrace TME 117 was resistant against CBSV from Kenya, it became infected with CBSV from Mozambique. Only the cassava genotype TMS/ 1089A revealed immunity against CMG and also did not establish infections with the CBSV isolates from diverse origins.

First report of cucumber mosaic virus in yams (Dioscorea spp.) in Ghana, Togo and Republic of Benin in West Africa, Eni, A., Kumar, P. L., Asiedu, R., Alabi, O., Naidu, R.*, Hughes, J. and Rey, M.*, in: Plant Disease, volume 92, number 5, pages 833, ISSN 0191-2917, 2008. [DOI]
 
Abstract:
Yam (Dioscorea spp., family Dioscoreaceae) is one of the most important food crops cultivated in the West African yam zone comprising the forest and savannah areas of Nigeria, Ghana, Cote d'Ivoire, Republic of Benin, and Togo, which account for more than 90% of the 4.59 million ha of yam cultivation worldwide (1). A survey was conducted in 2005 to document viruses in yams in Ghana, Togo, and the Republic of Benin. Samples (1,405) from five species of yam showing mosaic, chlorosis, and stunting as well as asymptomatic plants were tested for Dioscorea bacilliform virus (DBV, genus Badnavirus), Yam mosaic virus (YMV, genus Potyvirus), and Yam mild mosaic virus (YMMV, genus Potyvirus), the three most common viruses infecting yams. In addition, samples were tested for Cucumber mosaic virus (CMV), since CMV was previously reported to infect yams in Cote d'Ivoire (2) and Nigeria (3). In protein-A sandwich-ELISA with polyclonal antibodies to a cowpea isolate of CMV, 23 of the 1,405 samples (6 of 218 samples from Togo, 13 of 628 samples from Ghana, and 4 of 559 samples from Republic of Benin) tested positive for CMV. The CMV-positive samples were from D. alata (N = 16) and D. rotundata (N = 7), whereas all samples from D. cayenensis, D. dumetorum, and D. bulbifera tested negative. CMV was detected as mixed infections with DBV, YMV, or YMMV in 21 of 23 samples. Some of these samples showed puckering, chlorosis, mottling, and crinkling, whereas some plants infected by two or more viruses were asymptomatic. Only two samples from D. rotundata had a single infection of CMV and they showed mild chlorotic symptoms in young leaves that were inconspicuous in mature leaves. In sap inoculations, the virus induced systemic mosaic in Nicotiana glutinosa. The presence of CMV in ELISA-positive yam samples was further confirmed by immunocapture-reverse transcription (IC-RT)-PCR using CMV antibodies as trapping antibody and oligonucleotide primers specific for a 485 nt corresponding to 3' end of the coat protein gene and C-terminal noncoding region of RNA-3 (4). To confirm the specificity of ICRT-PCR, the 485-bp amplicons from an isolate from the Republic of Benin was cloned into pCR2.1 (Invitrogen, Carlsbad, CA) and three independent clones were sequenced from both orientations. Pairwise comparison of a consensus sequence (Accession No. EU274471) with corresponding sequences of other CMV isolates deposited in GenBank showed 99% identity at the nucleotide sequence level (Accession No. U22821) and revealed that the CMV isolate from yam belongs to sub-Group IA. To our knowledge, this is the first report of CMV infection in yams (D. alata and D. rotundata) in Ghana, Togo, and the Republic of Benin. Together with a previous documentation of CMV in D. alata and D. trifida in Cote d'Ivoire and Nigeria (2,3), this report adds to existing knowledge on distribution of CMV in yams with implications for yam production and germplasm distribution in the West Africa Region.

Novel sources of resistance to Fusarium stalk rot of maize in tropical Africa, Afolabi, C., Ojiambo, P., Ekpo, E.*, Menkir, A. and Bandyopadhyay, R., in: Plant Disease, volume 92, number 5, pages 772-780, ISSN 0191-2917, 2008.
 
Abstract:
Fusarium stalk rot is one of the most widespread and destructive diseases of maize, and deployment of resistant genotypes is one of the most effective strategies for controlling the disease. Fifty inbred lines and four checks from the breeding program of the International Institute of Tropical Agriculture were evaluated in field trials at Ikenne and Ibadan, Nigeria in 2003 and 2004 to identify new sources of resistance to stalk rot caused by Fusarium verticillioides. Evaluations were conducted under artificial inoculation and natural infection at Ibadan and Ikenne, respectively. Disease severity was recorded using a severity scale (SS) and direct estimation of stalk discoloration (SD). The two methods of disease assessment were compared and combined to classify genotypes into resistance groups using results from rank-sum analysis. In 2003, disease severity ranged from SS = 1 to 5 and SD = 1.3 to 33.8% at both locations. Both SS and SD were significantly (P < 0.01) higher in 2003 than in 2004 at the two locations. In both years, inbred lines significantly differed in SS (P < 0.02) and SD (P < 0.04) at Ibadan. Similarly, inbred lines significantly differed in SS (P < 0.04) and SD (P < 0.04) when genotypes were evaluated at Ikenne. Disease assessments based on SS and SD were significantly correlated (0.68 < r < 0.95, P < 0.01) in both years. Based on the results from rank-sum analysis, inbred lines were separated into highly resistant, resistant, moderately resistant, moderately susceptible, susceptible, and highly susceptible groups. At Ibadan, 6 (11.1%) and 8 (14.8%) were identified as highly resistant and resistant, respectively, whereas 11 (20.4%) were identified as resistant at Ikenne. Inbred lines 02C14609, 02C14643, 02C14654, and 02C14678 were consistently classified as either highly resistant or resistant to stalk rot across locations and years while the check genotypes were classified either as susceptible or moderately susceptible to stalk rot. These four inbred lines identified to have high levels of disease resistance may be used for breeding maize with resistance to Fusarium stalk rot.

Screenhouse and field persistence of nonpathogenic endophytic Fusarium oxysporum in Musa tissue culture plants, Paparu, P., Dubois, T., Gold, C., Adipala, E.*, Niere, B. and Coyne, D., in: Microbial Ecology, volume 55, number 3, pages 561-568, ISSN 1432-184X, 2008.
 
Abstract:
Two major biotic constraints to highland cooking banana (Musa spp., genome group AAA-EA) production in Uganda are the banana weevil Cosmopolites sordidus and the burrowing nematode Radopholus similis. Endophytic Fusarium oxysporum strains inoculated into tissue culture banana plantlets have shown control of the banana weevil and the nematode. We conducted screenhouse and field experiments to investigate persistence in the roots and rhizome of two endophytic Fusarium oxysporum strains, V2w2 and III4w1, inoculated into tissue-culture banana plantlets of highland cooking banana cultivars Kibuzi and Nabusa. Re-isolation of F. oxysporum showed that endophyte colonization decreased faster from the rhizomes than from the roots of inoculated plants, both in the screenhouse and in the field. Whereas rhizome colonization by F. oxysporum decreased in the screenhouse (4-16 weeks after inoculation), root colonization did not. However, in the field (17-33 weeks after inoculation), a decrease was observed in both rhizome and root colonization. The results show a better persistence in the roots than rhizomes of endophytic F. oxysporum strains V2w2 and III4w1.