| Assessment of RNAi-induced silencing in banana (Musa spp), Dang, T. V. T., Windelinckx, S., Henry, I. M., De Coninck, B., Cammue, B. P. A., Swennen, R. and Remy, S., in: BMC Research Notes, volume 7, number 655, pages 1-30, ISSN 1756-0500, 2014. [DOI] |
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| Cassava improvement in Africa: contribution of the Internaitonal Institute of Tropical Agriculture, Dixon, A., Asiedu, R., Ekanayake, I. J. and Porto, M. C. M., in: Proceedings African Crop Science Conference, pages 466-469, African Crop Science Society, 1994. |
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| Development of transgenic banana and plantains using RNAi Approach for control of banana aphids, Jekayinoluwa, T. A.*, University of Nairobi, 2020. |
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| Identifying aflatoxin resistance-related proteins/genes through proteomics and RNAi gene silencing, Chen, Z. Y., Brown, R., Guo, B. Z., Menkir, A. and Cleveland, T. E., in: Peanut Science, volume 36, number 1, pages 35-41, ISSN 0095-3679, 2009. |
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| RNAi technology for management of banana bunchy top disease, Jekayinoluwa, T., Tripathi, L., Tripathi, J., Ntui, V. O., Obiero, G.*, Muge, E.* and Dale, J., in: Food and Energy Security, pages 1-15, ISSN 2048-3694, 2020. [DOI] |
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| Assessment of RNAi-induced silencing in banana (Musa spp), Dang, T. V. T., Windelinckx, S., Henry, I. M., De Coninck, B., Cammue, B. P. A., Swennen, R. and Remy, S., in: BMC Research Notes, volume 7, number 655, pages 1-30, ISSN 1756-0500, 2014. [DOI] |
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| Keywords: | Bananas; RNAi; Musa
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| Identifying aflatoxin resistance-related proteins/genes through proteomics and RNAi gene silencing, Chen, Z. Y., Brown, R., Guo, B. Z., Menkir, A. and Cleveland, T. E., in: Peanut Science, volume 36, number 1, pages 35-41, ISSN 0095-3679, 2009. |
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Abstract:
Aflatoxins are carcinogenic secondary metabolites
produced mainly by Aspergillus flavus Link ex. Fries, and A. prarasiticus Speare during infection of susceptible crops, such as maize, cottonseed, peanuts and tree nuts. This paper will review research efforts in identifying aflatoxin resistance-related proteins/genes in maize. Similar strategies may be useful in peanut. For maize, although genotypes resistant to A. flavus infection or aflatoxin production have been identified, the incorporation of resistance into commercial lines has been slow due to the lack of selectable markers
and poor understanding of host resistance mechanisms. Recently, resistance-associated proteins (RAPs) were identified through proteomic comparison of constitutive protein profiles between
resistant and susceptible maize genotypes. These
proteins belong to three major groups based on
their peptide sequence homologies: storage proteins, stress-related proteins, and antifungal proteins. Preliminary characterization of some of
these RAPs suggest that they play a direct role in
host resistance, such as pathogenesis-related
protein 10 (PR10), or an indirect role, such as
glyoxalase I (GLX I), through enhancing the host
stress tolerance. To verify whether these RAPs
play a role in host resistance, RNA interference
(RNAi) gene silencing technique was used to
silence the expression of these genes in maize.
RNAi vectors (glx I RNAi and pr10 RNAi) were
constructed using Gateway technology, and then
transformed into immature maize embryos using
both bombardment and Agrobacterium infection.
The extent of gene silencing in transgenic callus
tissues ranged from 20% to over 99%. The RNAi
silenced transgenic maize seeds have also been
obtained from plants regenerated from Agrobacterium transformed callus lines. Kernel screen assay of the transgenic maize kernels demonstrated a significant increase in susceptibility to A. flavus colonization and aflatoxin production in
some of the silenced transgenic lines compared
with non-silenced control kernels, suggesting the
direct involvement of these two proteins in
aflatoxin resistance in maize.
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