| Evaluation of cowpea (Vigna unguiculata (L.) Walp.) landraces to bacterial blight caused by Xanthomonas axonopodis pv. vignicola, Durojaye, H. A., Moukoumbi, Y. D., Dania, V. O.*, Boukar, O., Bandyopadhyay, R. and Ortega-Beltran, A., in: Crop Protection, volume 116, pages 77-81, ISSN 0261-2194, 2019. [DOI] |
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| Genetic diversity among Xanthomonas axonopodis pv Manihotis isolated from the western states of Nigeria, Ogunjobi, A.*, Dixon, A. and Fagade, O.*, in: Book of Abstracts of the 9th Triennial Symposium of the International Society for Tropical Root Crops-Africa Branch, 31 October-5 November 2004, Mombasa, Kenya, 2004. |
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| Genetic diversity among Xanthomonas axonopodis pv manihotis isolated from the western states of Nigeria, Ogunjobi, A.*, Dixon, A. and Fagade, O.*, in: Proceedings of 9th ISTRC-AB symposium, pages 557-563, Mombasa, Kenya, 2007. |
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| Genotype x environment effects on severity of cassava bacterial blight disease caused by Xanthomonas axonopodis pv. manihotis, Dixon, A., Ngeve, J. and Nukenine, E., in: European Journal of Plant Pathology, volume 108, number 8, pages 763-770, 2002. |
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| Molecular variation in population structure of Xanthomonas axonopodis pv Manihotis in the south eastern Nigeria, Ogunjobi, A.*, Fagade, O.* and Dixon, A., in: African Journal of Biotechnology, volume 5, number 20, pages 1868-1872, ISSN 1684-5315, 2006. |
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| Physiological studies on Xanthomonas axonopodis pv Manihotis (Xam) strains isolated in Nigeria, Ogunjobi, A.*, Fagade, O.* and Dixon, A., in: Electronic Journal of Environmental, Agricultural and Food Chemistry, volume 6, number 10, pages 2482-2489, ISSN 1579-4377, 2007. |
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| Prevalence and severity of bacterial blight and anthracnose diseases of cassava in different agro-ecological zones of Nigeria, Onyeka, T., Owolade, F., Ogunjobi, A.*, Dixon, A., Okechukwu, R., Bandyopadhyay, R. and Bamkefa, B., in: African Journal of Agricultural Research, volume 3, number 4, pages 297-304, ISSN 1991-637X, 2008. |
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| Keywords: | anthracnose, bacterial blight, cassava, Colletotrichum gloeosporioides prevalence, severity, Xanthomonas axonopodis
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| Development of a semi-selective medium for isolating Xanthomonas camprestris pv. musacearum from insect vectors, infected plant material and soil, Mwangi, M., Mwebaze, M., Bandyopadhyay, R., Aritua, V.*, Eden-Green, S., Tushemereirwe, W. K.* and Smith, J., in: Plant Pathology, volume 56, number 3, pages 383-390, ISSN 0032-0862, 2007. |
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Abstract:
A semiselective medium was developed for isolating Xanthomonas campestris pv. musacearum (Xcm) from infected banana plants, soil and insect vectors. The new medium was named cellobiose-cephalexin agar (CCA) and it contained (L{\^a}ˆ’1): 1 g yeast extract, 1 g glucose, 1 g peptone, 1 g NH4Cl, 1 g MgSO4{\"i}¿½7H2O, 3 g K2HPO4, 1 g beef extract, 10 g cellobiose, 14 g agar, 40 mg cephalexin, 10 mg 5-fluorouracil and 120 mg cycloheximide. The medium was evaluated for selectivity using 21 bacterial isolates and for plating efficiency using Xcm. The bacterial isolates included a soilborne Xanthomonas species and three pathogenic Xanthomonas strains that infect cassava, cabbage and beans. Although the plating efficiency of Xcm on CCA was lower (59%) than on non-selective yeast extract peptone glucose agar (YPGA), its selectivity was significantly higher, averaging 60 and 82%, when isolating from banana fruits and soil, respectively. CCA was also superior when isolating Xcm from insect vectors, with selectivity of 48-75%, compared with 8-17% on YPGA. Xanthomonas campestris pv. phaseoli did not grow on CCA, while X. campestris pv. campestris and X. axonopodis pv. manihotis grew, but their colonies were smaller than those of Xcm. Twenty-nine out of 33 suspected Xcm strains isolated from plants, soil and insects using CCA were pathogenic when inoculated onto banana plants, indicating that CCA can be a reliable tool in isolating Xcm populations. The medium should prove useful in studies on ecology, epidemiology and management of the banana bacterial wilt pathogen that is currently ravaging bananas in East and Central Africa.
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