Titles (18)Keywords (1)Abstracts (1)
A sensitive TAS-ELISA for the detection of some West African isolates of yam mosaic virus (YMV) in Dioscorea species, Njukeng, A. P.*, Atiri, G.*, Hughes, J., Agindotan, B., Mignouna, H. and Thottappilly, G., in: Tropical Science, volume 42, pages 65-74, 2002.
 
Assessment of yam mild mosaic virus coat protein gene sequence diversity reveals the prevalence of cosmopolitan and African group of isolates in Ghana and Nigeria, Nkere, C., Otoo, E.*, Atiri, G.*, Onyeka, J.*, Silva, G., Bomer, M., Seal, S. and Kumar, P. L., in: Current Plant Biology, number 100156, pages 1-6, ISSN 2214-6628, 2020. [DOI]
 
Characterization of cucumber mosaic virus isolated from yam (Dioscorea spp.) in West Africa, Eni, A*, Kumar, P. L., Asiedu, R., Alabi, O., Naidu, R., Hughes, J.+ and Rey, M.E.C., in: African Journal of Biotechnology, volume 12, number 22, pages 3472-3480, ISSN 1684-5315, 2013. [DOI]
 
Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP), Nkere, C., Oyekanmi, J., Silva, G., Bomer, M., Atiri, G.*, Onyeka, J.*, Maroya, N., Seal, S. and Kumar, P. L., in: Archives of Virology, pages 1-10, ISSN 0304-8608, 2018. [DOI]
 
Comparative sensitivity of triple-antibody sandwich enzyme-linked immunosorbent assay(TAS-ElISA) and immunocapture-reverse transcription polymerase chain reaction (IC-RT-PCR) for the detection of yam mosaic virus, genus Polyvirus, in yam, Hughes, J., Olatunde, O., Canning, E., Seal, S., Kenyon, L. and Offei, S.*, in: Book of Abstracts of the Eighteenth Annual Conference of the Nigerian Institute of Science Technology, 28 Nov-1 Dec. 2000, Maiduguri, Nigeria, pages 25-26, 2000.
 
Efficient elimination of Yam mosaic virus (YMV) from white yam (Dioscorea rotundata Poir.) by cryotherapy of axillary buds, Ita, E. E.*, Uyoh, E. A.*, Nakamura, I. and Ntui, V. O., in: South African Journal of Botany, volume 130, pages 123-129, ISSN 0254-6299, 2020. [DOI]
 
Epidemiological analysis of yam mosaic virus (YMV) infection risk factors and its implications for clean seed yam systems: poster, Osundahunsi, B., Oviasuyi, T., Odu, B. O.*, Aighewi, B. and Kumar, P. L., International Institute of Tropical Agriculture, 2022.
 
Evaluation of resistance to yam mosaic virus (YMV), genus Potyvirus in white yam (Dioscorea rotundata Poir.), Odu, B., Asiedu, R., Hughes, J., Shoyinka, S.* and Oladiran, O., in: Field Crops Research, volume 89, pages 97-105, 2004.
 
Exploring quantitative trait nucleotides associated with response to yam mosaic virus severity and tuber yield traits in Dioscorea praehensilis Benth. germplasm via genome-wide association scanning, Adewumi, A. S., Adejumobi, I. I., Opoku, V. A.*, Asare, P. A.*, Adu, M. O.*, Taah, K. J.*, Stanley, A. E.*, Olatunde, T. E.*, Afutu, E.*, Akaba, S.*, Mushoriwa, H . and Agre, A. P., in: Frontiers in Horticulture, volume 3, number - :1459476, pages 1-15, ISSN 2813-3595, 2024. [DOI]
 
First report of cucumber mosaic virus in yams (Dioscorea spp.) in Ghana, Togo and Republic of Benin in West Africa, Eni, A., Kumar, P. L., Asiedu, R., Alabi, O., Naidu, R.*, Hughes, J. and Rey, M.*, in: Plant Disease, volume 92, number 5, pages 833, ISSN 0191-2917, 2008. [DOI]
 
Genome-wide mapping uncovers significant quantitative trait loci associated with yam mosaic virus infection, yield and dry matter content in White Guinea yam (Dioscorea rotundata Poir.), Adjei, E. A.*, Odong, T. L.*, Esuma, W.*, Bhattacharjee, R., Agre, A. P., Adebola, P. O., Chamba, E.*, Asfaw, A., Dramadri, I.*, Mbabazi, S. T.*, Edema, R.*, Ozimati, A.*, Ochwo-Ssemakula, M.* and Alicai, T.*, in: Frontiers in Horticulture, volume 3, number -: 1365567, pages 1-13, ISSN 2813-3595, 2024. [DOI]
 
Identification and potential use of RAPD markers linked to yam mosaic virus resistance in white yam (Dioscorea rotundata Poir.), Mignouna, H., Abang, M., Onasanya, A., Agindotan, B. and Asiedu, R., in: Annals of Applied Biology, volume 140, pages 163-169, 2002.
 
Identification of quantitative trait nucleotides and candidate genes for tuber yield and mosaic virus tolerance in an elite population of white guinea yam (Dioscorea rotundata) using genome-wide association scan, Agre, A. P., Norman, P. E.*, Asiedu, R. and Asfaw, A., in: BMC Plant Biology, volume 21, number : 552, pages 1-16, ISSN 1471-2229, 2021. [DOI]
 
Inheritance of resistance to yam mosaic virus, genus Potyvirus, in white yam (Dioscorea rotundata), Mignouna, H., Njukeng, A. P.*, Abang, M. and Asiedu, R., in: Theoretical and Applied Genetics, volume 103, pages 1196-2000, 2001.
 
Potential of West African white yam (Dioscorea rotundata Poir.) genotypes as sources of resitance to yam mosaic virus (genus Potyvirus; family Potyviridae), Odu, B., Asiedu, R., Hughes, J., Shoyinka, S.* and Oladiran, A., 2001.
 
Rapid and specific detection of Yam mosaic virus by reverse-transcription recombinase polymerase amplification, Silva, G., Bomer, M., Nkere, C., Kumar, P. L. and Seal, S., in: Journal of Virological Methods, volume 222, pages 138-144, ISSN 0166-0934, 2015. [DOI]
 
Re-evaluation of Yam Mosaic Virus (YMV) detection methods, Eni, A*, Hughes, J., Asiedu, R. and Rey, M.*, in: Academic Journal of Plant Sciences, volume 5, number 1, pages 18-22, ISSN 1995-8986, 2012. [DOI]
 
Transgenic tobacco lines expressing yam mosaic virus coat protein-derived dsRNA are resistant to yam mosaic virus, Ita, E. E.*, Uyoh, E. A.*, Nakamura, I. and Ntui, V. O., in: American Journal of Plant Sciences, volume 11, number 9, pages 1487-1504, ISSN 2158-2742, 2020. [DOI]
 
Environmental stability of resistance to anthracnose and virus diseases of water yam (Dioscorea alata), Egesi, C. N.*, Onyeka, T. J.* and Asiedu, R., in: African Journal of Agricultural Research, volume 4, number 2, pages 113-118, ISSN 1991-637X, 2009.
 
Keywords:Colletotrichum gloeosporioides; genotype x environment (G x E) interaction; stable resistance; water yam; yam anthracnose; yam mosaic virus

First report of cucumber mosaic virus in yams (Dioscorea spp.) in Ghana, Togo and Republic of Benin in West Africa, Eni, A., Kumar, P. L., Asiedu, R., Alabi, O., Naidu, R.*, Hughes, J. and Rey, M.*, in: Plant Disease, volume 92, number 5, pages 833, ISSN 0191-2917, 2008. [DOI]
 
Abstract:
Yam (Dioscorea spp., family Dioscoreaceae) is one of the most important food crops cultivated in the West African yam zone comprising the forest and savannah areas of Nigeria, Ghana, Cote d'Ivoire, Republic of Benin, and Togo, which account for more than 90% of the 4.59 million ha of yam cultivation worldwide (1). A survey was conducted in 2005 to document viruses in yams in Ghana, Togo, and the Republic of Benin. Samples (1,405) from five species of yam showing mosaic, chlorosis, and stunting as well as asymptomatic plants were tested for Dioscorea bacilliform virus (DBV, genus Badnavirus), Yam mosaic virus (YMV, genus Potyvirus), and Yam mild mosaic virus (YMMV, genus Potyvirus), the three most common viruses infecting yams. In addition, samples were tested for Cucumber mosaic virus (CMV), since CMV was previously reported to infect yams in Cote d'Ivoire (2) and Nigeria (3). In protein-A sandwich-ELISA with polyclonal antibodies to a cowpea isolate of CMV, 23 of the 1,405 samples (6 of 218 samples from Togo, 13 of 628 samples from Ghana, and 4 of 559 samples from Republic of Benin) tested positive for CMV. The CMV-positive samples were from D. alata (N = 16) and D. rotundata (N = 7), whereas all samples from D. cayenensis, D. dumetorum, and D. bulbifera tested negative. CMV was detected as mixed infections with DBV, YMV, or YMMV in 21 of 23 samples. Some of these samples showed puckering, chlorosis, mottling, and crinkling, whereas some plants infected by two or more viruses were asymptomatic. Only two samples from D. rotundata had a single infection of CMV and they showed mild chlorotic symptoms in young leaves that were inconspicuous in mature leaves. In sap inoculations, the virus induced systemic mosaic in Nicotiana glutinosa. The presence of CMV in ELISA-positive yam samples was further confirmed by immunocapture-reverse transcription (IC-RT)-PCR using CMV antibodies as trapping antibody and oligonucleotide primers specific for a 485 nt corresponding to 3' end of the coat protein gene and C-terminal noncoding region of RNA-3 (4). To confirm the specificity of ICRT-PCR, the 485-bp amplicons from an isolate from the Republic of Benin was cloned into pCR2.1 (Invitrogen, Carlsbad, CA) and three independent clones were sequenced from both orientations. Pairwise comparison of a consensus sequence (Accession No. EU274471) with corresponding sequences of other CMV isolates deposited in GenBank showed 99% identity at the nucleotide sequence level (Accession No. U22821) and revealed that the CMV isolate from yam belongs to sub-Group IA. To our knowledge, this is the first report of CMV infection in yams (D. alata and D. rotundata) in Ghana, Togo, and the Republic of Benin. Together with a previous documentation of CMV in D. alata and D. trifida in Cote d'Ivoire and Nigeria (2,3), this report adds to existing knowledge on distribution of CMV in yams with implications for yam production and germplasm distribution in the West Africa Region.